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Figure 8 - from "Establishing tissue-specific chromatin organization during development of the epidermis. Nuclear architecture of different layers of murine epidermis and the role of p63 and Satb1 in establishing tissue-specific organization of the epidermal differentiation complex locus"

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arrow_backarrow_forwardFigure 1.9 Different models explaining the distribution and organization of chromosomes within the interphase nucleus. A - interchromatin domain model (ICD) — impenetrable for high molecular mass particles chromosome territories are separated by DNA free “interchromatin domain”; B - chromosome territory — interchrimatin compartment (CT-IT) — modification of the previous model, which assumes that chromosomes are intersected by channels and the invaginations of the surface of chromosome territories; C,D - interchromosomal network model (ICM) — chromosome territory is a network occupying some particular space but intermingling with neighboring chromosome territories(Branco and Pombo, 2007). chromosomal network model (ICN) (Branco and Pombo, 2007). molecular mass particles chromosome territories are separated by DNA free “interchromatin domain”; B - chromosome territory — interchrimatin compartment (CT-IT) — modification of the

Figure 1.9 Different models explaining the distribution and organization of chromosomes within the interphase nucleus. A - interchromatin domain model (ICD) — impenetrable for high molecular mass particles chromosome territories are separated by DNA free “interchromatin domain”; B - chromosome territory — interchrimatin compartment (CT-IT) — modification of the previous model, which assumes that chromosomes are intersected by channels and the invaginations of the surface of chromosome territories; C,D - interchromosomal network model (ICM) — chromosome territory is a network occupying some particular space but intermingling with neighboring chromosome territories(Branco and Pombo, 2007). chromosomal network model (ICN) (Branco and Pombo, 2007). molecular mass particles chromosome territories are separated by DNA free “interchromatin domain”; B - chromosome territory — interchrimatin compartment (CT-IT) — modification of the

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Figure. 1.1 Epidermal morphogenesis in wild type mouse and p63 knock out mouse. After commitment to epidermal fate, the basal layer cells give rise to periderm that sheds in later stages, subsequently the intermediate layer of cells appears between them which undergoes maturation into spinous cells. Spinous cells differentiate into the granular layer and eventually cornified layer. The whole process of epidermal stratification leads to the establishment of the epidermal barrier which is inevitable for vitality of mouse. p63 knock out mouse dies shortly after birth from dehydration and often abnormalities (Koster and Roop, 2007b). functional multilayered epidermis at E18.5 (M'Boneko and Merker, 1988).
Figure 1.2 Skin morphogenesis and its regulation. Explanation in the text (adapted from Koster and Roop, 2007) (Hoffjan and Stemmler, 2007). establishing a lipid envelope that further reinforces the epidermal barrier The epidermal barrier is indispensible for survival of the newborn mice. p63
Figure 1.4 Nucleus and its suborganelles (Spector, 2003). Nucleus is a complex organelle of eukaryotic cells storing and processing genetic information, participating in ribosome biogenesis and in many other processes. It is filled with chromosome territories occupying individual spaces. Also, there are plethora of nuclear bodies differing in their properties and functions saan hetween the chromosome territories eukaryotic cells storing and processing genetic information, participating in ribosome biogenesi:
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Figure 1.5. Chromatin fibre is a mix of zigzag and solenoid structure — scheme of EMANIC experiment (Woodcock and Ghosh). arrangement interrupted by solenoidal structure (Grigoryev et al., 2009). analysed) suggested 30 nm fibre as a heteromorphus structure with the zigzag
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Figure 1.6 Organisation of heterochromatin in the nucleus. (A) Schematic illustration of the interphase nucleus of a mouse B cell. Condensed clusters of pericentromeric heterochromatin, each containing several centromeres. (B) In differentiated post-mitotic cells such as plasma cells and glial cells, much of the genome becomes condensed into facultative heterochromatin. The detailed organisation of this type of heterochromatin remains unknown. (C) Regions of intercalary heterochromatin have been described on the long arms of Drosophila chromosomes (Dillon, 2004).
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Michal Gdula
Michal Gdula
Establishing tissue-specific chromatin organization during development of the epidermis. Nuclear architecture of different layers of murine epidermis and the role of p63 and Satb1 in establishing tissue-specific organization of the epidermal differentiation complex locus

Abstract: During development, multipotent stem cells establish tissue-specific programmes of gene expression that underlie a process of differentiation into specialized cell types. It was shown in the study that changes in the nuclear architecture during terminal keratinocyte differentiation show correlation with the dynamics of the transcriptional and metabolic activity. In particular, terminal differentiation is accompanied by the decrease of nuclear volume, elongation of its shape, reduction of the number and fusion of nucleoli, increase in the number of centromeric clusters and a dramatic decrease of the transcriptional activity. Global changes in the nuclear architecture of epidermal keratinocytes are associated with marked remodelling of the higher-order chromatin structure of the epidermal differentiating complex (EDC). EDC is positioned peripherally in the epidermal nuclei at E11.5 when its genes show low expression levels and relocates towards the nuclear interior at E16.5 when EDC g...

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