Papers by Hasanuzzaman Talukder BAU
Journal of the Bangladesh Agricultural University, 2017
Research Square (Research Square), Feb 19, 2024
Fascioliasis is one of the most common foodborne zoonotic infection of ruminants in Bangladesh. T... more Fascioliasis is one of the most common foodborne zoonotic infection of ruminants in Bangladesh. To estimate the prevalence and associated risk factors of fascioliasis and extent of liver damage, 825 livers of sheep and goats were randomly inspected during onsite slaughterhouse visiting in Naogaon, Natore, Rajshahi and Joypurhat districts. The overall prevalence of fascioliasis was 25.09% and signi cantly (P = 0.008) higher in goats (26.11%) than sheep (24.00%). During gross inspection, Fasciolainfected livers were increased in size, brosed, fatty, multiple white or reddish necrotic foci on the parietal surface, hard to cut, calci ed, and numerous mature and immature ukes were also observed. In histoarchitecture, in ammatory cell in ltration in the hepatic parenchyma and periportal area, brous connective tissue proliferation around necrotic area, hyperplastic bile duct, congestion, and primary biliary cirrhosis were seen in acute and chronic fascioliasis. Epidemiological investigations revealed that fascioliasis was higher in goats than sheep. Age, sex, BCS and season were found to have statistically signi cant associations with fascioliasis in goats.
Haemonchus contortus, an obligatory haematophagus worm infection in small ruminants: Population genetics and genetic diversity
Al-Mi’galaẗ al-sa'udiyaẗ lī-ulum al-ḥayaẗ, May 1, 2024
Molecular and phylogenetic characterization of zoonotic <i>Trichostrongylus</i> species from goats for the first time in Bangladesh
Transactions of The Royal Society of Tropical Medicine and Hygiene, Jun 13, 2023
Background Trichostrongylus is one of the most important nematodes infecting animals and humans. ... more Background Trichostrongylus is one of the most important nematodes infecting animals and humans. The current study was designed to identify the Trichostrongylus species infecting goats by multiplex PCR and phylogenetic analysis. Methods A total of 124 goats’ viscera were collected from different abattoirs of Mymensingh division. Trichostrongylus species were isolated and characterized based on morphometry, multiplex PCR and phylogenetic analysis. Results Among 124 viscera of goats, 39 were positive with two species, Trichostrongylus colubriformis and Trichostrongylus vitrinus, revealing an overall 31.45% prevalence. Morphological identification of Trichostrongylus species was confirmed by multiplex PCR amplification of the ITS2 gene and sequencing. Partial sequencing of the ITS2 gene of two species revealed seven single nucleotide polymorphisms (three transitions and four transversions) in this study. The neighbor-joining phylogenetic tree demonstrated that T. colubriformis and T. v...
Veterinary Parasitology: Regional Studies and Reports, 2018
This report aimed to assess the seroprevalence of Toxoplasma gondii infection in 708 swine matric... more This report aimed to assess the seroprevalence of Toxoplasma gondii infection in 708 swine matrices in Nova Mutum and Diamantino in the state of Mato Grosso, Central-West Brazil. Serum samples were examined by indirect fluorescent antibody test (IFAT). It was found a seroprevalence of 12.8%, considering titers ≥64. Therefore, the data reinforce the need for appropriate management of swine raising to minimize the risk of infection of pigs with T. gondii.
PubMed, Oct 1, 2009
This study aimed to investigate and compare the antagonistic effects of atipamezole and yohimbine... more This study aimed to investigate and compare the antagonistic effects of atipamezole and yohimbine on medetomidine-induced diuresis in healthy dogs. Five dogs were used repeatedly in each of 8 groups. One group was not medicated. Dogs in the other groups received 20 microg/kg of medetomidine intramuscularly and, 0.5 h later, saline (as the control injection), 50, 100, or 300 microg/kg of atipamezole, or 50, 100, or 300 microg/kg of yohimbine intramuscularly. Urine and blood samples were taken 11 times over 24 h for measurement of the following: urine volume, specific gravity, and creatinine concentration; urine and plasma osmolality; urine and plasma concentrations of electrolytes and arginine vasopressin (AVP); and the plasma concentration of atrial natriuretic peptide (ANP). Both atipamezole and yohimbine antagonized the diuretic effect of medetomidine, inhibiting medetomidine-induced decreases in urine specific gravity, osmolality, and concentrations of creatinine, sodium, potassium, chloride, and AVP and reversing both the medetomidine-induced increase in plasma concentrations of sodium, potassium, and chloride and the medetomidine-induced decrease in the plasma AVP concentration. Atipamezole significantly stimulated ANP release. The antidiuretic action of yohimbine was more potent than that of atipamezole but was not dose-dependent, in contrast to the action of atipamezole. The effects of these drugs may not be due only to actions mediated by alpha(2)-adrenoceptors.
Model Nematodes as a Practical Innovation to Promote High Throughput Screening of Natural Products for Anthelmintics Discovery in South Asia: Current Challenges, Proposed Practical & Conceptual Solutions
Journal of the Bangladesh Agricultural University, Sep 30, 2019
Gastrointestinal nematode causes significant production losses in grazing ruminants and anthelmin... more Gastrointestinal nematode causes significant production losses in grazing ruminants and anthelmintic resistance are increasing at an alarming rate. The aim of this study was to evaluate the in vitro anthelmintic effects of tannin-containing plant Artemisia vulgaris against stomach worm Haemonchus contortus of goat. In this respect, in vitro anthelmintic activities of crude aqueous and ethanolic extracts of aerial parts of A. vulgaris were investigated on eggs, larvae (L3) and adults of H. contortus in the laboratory. Experimental plant Artemisia was collected, dried in shade and grinded to coarse powder and subjected to prepare crude aqueous and ethanolic extract. The experiment revealed that crude ethanolic extracts completely inhibited egg hatching at concentration of 5 mg/ml whereas aqueous extracts at concentration of 10 mg/ml. After exposure of 8 hours, the crude ethanolic extracts inhibited 69.33%, 51% and 38% larval motility tested at 20 mg/ml, 10 mg/ml and 5 mg/ml concentrations, respectively while the crude aqueousextracts inhibited 57.33%, 48.67% and 34.67% at the same concentrations. The ethanolic extract showed better in vitro effects against adult stomach worm than the aqueous extract in terms of motility inhibition at different hours post-treatment. Dose dependent effects were also observed for both extract. After 2 and 8 hours of exposure, the ethanolic extract induced 53.33% and 100% mortality at the highest tested concentration respectively, while the aqueous extract induced 47.67% and 86.67% at the same concentration. To our best knowledge, this is the first study in Bangladesh on the in vitro anthelmintic effects of A. vulgaris against H. contortus. Further in vitro and in vivo trials with this plant are required to evaluate their anthelmintic effects precisely.
Journal of extracellular vesicles, Jan 21, 2018
Whipworms are parasitic nematodes that live in the gut of more than 500 million people worldwide.... more Whipworms are parasitic nematodes that live in the gut of more than 500 million people worldwide. Owing to the difficulty in obtaining parasite material, the mouse whipworm Trichuris muris has been extensively used as a model to study human whipworm infections. These nematodes secrete a multitude of compounds that interact with host tissues where they orchestrate a parasitic existence. Herein we provide the first comprehensive characterization of the excretory/secretory products of T. muris. We identify 148 proteins secreted by T. muris and show for the first time that the mouse whipworm secretes exosome-like extracellular vesicles (EVs) that can interact with host cells. We use an Optiprep® gradient to purify the EVs, highlighting the suitability of this method for purifying EVs secreted by a parasitic nematode. We also characterize the proteomic and genomic content of the EVs, identifying >350 proteins, 56 miRNAs (22 novel) and 475 full-length mRNA transcripts mapping to T. muris gene models. Many of the miRNAs putatively mapped to mouse genes are involved in regulation of inflammation, implying a role in parasite-driven immunomodulation. In addition, for the first time to our knowledge, colonic organoids have been used to demonstrate the internalization of parasite EVs by host cells. Understanding how parasites interact with their host is crucial to develop new control measures. This first characterization of the proteins and EVs secreted by T. muris provides important information on whipworm-host communication and forms the basis for future studies.
Molecular and phylogenetic characterization of zoonotic Trichostrongylus species from goats for the first time in Bangladesh
Transactions of The Royal Society of Tropical Medicine and Hygiene
Background Trichostrongylus is one of the most important nematodes infecting animals and humans. ... more Background Trichostrongylus is one of the most important nematodes infecting animals and humans. The current study was designed to identify the Trichostrongylus species infecting goats by multiplex PCR and phylogenetic analysis. Methods A total of 124 goats’ viscera were collected from different abattoirs of Mymensingh division. Trichostrongylus species were isolated and characterized based on morphometry, multiplex PCR and phylogenetic analysis. Results Among 124 viscera of goats, 39 were positive with two species, Trichostrongylus colubriformis and Trichostrongylus vitrinus, revealing an overall 31.45% prevalence. Morphological identification of Trichostrongylus species was confirmed by multiplex PCR amplification of the ITS2 gene and sequencing. Partial sequencing of the ITS2 gene of two species revealed seven single nucleotide polymorphisms (three transitions and four transversions) in this study. The neighbor-joining phylogenetic tree demonstrated that T. colubriformis and T. v...
Author response for "Molecular detection of Babesia and Theileria from crossbred cattle in Sirajganj and Rangpur districts of Bangladesh
Laboratory studies on the life cycle of Boophilus microplus on rabbit
Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária
Anthelmintic resistance is an increasing problem that threatens livestock production worldwide. U... more Anthelmintic resistance is an increasing problem that threatens livestock production worldwide. Understanding of the genetic basis of benzimidazole resistance recently allowed the development of promising molecular diagnostic tools. In this study, isolates of Haemonchus contortus obtained from goats, sheep and buffaloes raised in Brazil were screened for presence of the polymorphism Phe200Tyr in the β-tubulin 1 gene, which confers resistance to benzimidazole. The allelic frequency of the mutation conferring resistance ranged from 7% to 43%, and indicated that resistance to benzimidazole could be found in nematodes isolated from all the ruminant species surveyed. Although significant variation in the frequency of the F200Y mutation was observed between different herds or host species, no significant variation could be found in populations isolated from animals within the same herd. These findings suggest that screening of samples from a few animals has the potential to provide inform...
Ex-vivo efficacy of commercially available anthelmintics against blood feeding stomach worm Haemonchus contortus of ruminants
Journal of Parasitic Diseases, Jan 10, 2023
Molecular detection of Babesia and Theileria from crossbred cattle in Sirajganj and Rangpur districts of Bangladesh
Veterinary Medicine and Science
Research in Agriculture Livestock and Fisheries
Surveillance was conducted to investigate the ecto-and endo-parasitic (gastrointestinal) infectio... more Surveillance was conducted to investigate the ecto-and endo-parasitic (gastrointestinal) infections of bapard cattle farm from July 2020 to June 2021 at the Laboratories of BAPARD and Department of Parasitology, BAU. In the study area, a total number of 600 fecal samples and 250 ectoparasites were collected and investigated. Fecal samples were examined by direct smear method followed by McMaster counting technique under microscope. The overall prevalence of endoparasitic (gastrointestinal) infections was 67.5% and ectoparasitic infection was 56%. Prevalence of endoparasite was more frequent in rainy season (52.35%) followed by summer (26.91%) and winter season (20.74%) whereas prevalence of ectoparasite was more prevalent in summer (34.00%) followed by rainy (12.00%) and winter (8.00%) season. Among the endoparasitic infections,high prevalence of Paramphistomum spp. (20.00%) was found in rainy season whereas Haemonchus spp. (11.67%) and Toxocara spp. (10.83%) were higher in summer s...
Trichostrongylus is one of the most economically important and zoonotic trichostrongylid nematode... more Trichostrongylus is one of the most economically important and zoonotic trichostrongylid nematode infecting both animals and humans, causing a disease known as trichostrongyliasis. The study was designed to identify Trichostrongylus species infecting goats by morphology-morphometry followed by multiplex PCR and phylogenetic characterization. A total of 124 viscera of goats were collected from different slaughter houses of Mymensingh division of Bangladesh, processed and trichostrongylids were isolated and identified under microscope. Among 124 viscera of goats, 39 were positive with two species of Trichostrongylus, namely, T. colubriformis and T. vitrinus showing an overall 31.45% prevalence. Morphological identification of Trichostrongylus species was confirmed by multiplex PCR amplification of ITS2 gene and sequencing (accession numbers ON721586 to ON721593 for T. colubriformis and T. vitrinus, respectively). Partial sequencing of ITS2 gene of T. vitrinus and T. colubriformis show...
Efficacy of flukicides on Fasciola gigantica, a food-borne zoonotic helminth affecting livestock in Bangladesh
Parasitology
Fasciola gigantica, the causative agent of tropical fasciolosis, is a food-borne zoonotic tremato... more Fasciola gigantica, the causative agent of tropical fasciolosis, is a food-borne zoonotic trematode that affects around 80% livestock of Bangladesh. Triclabendazole (TCBZ), nitroxynil (NTON) and oxyclozanide (OCZN) are frequently used against fascioliasis; however, the current status of potency of these flukicides was unknown. In this study, in vitro efficacy of TCBZ, NTON and OCZN at various concentrations on F. gigantica has been evaluated by relative motility (RM), morphological distortions of apical cone through an inverted microscope, architectural and ultra-structural changes through histopathological and scanning electron microscopy (SEM). It is observed that TCBZ, NTON and OCZN at higher concentrations significantly (P < 0.05) reduced RM of the flukes compared to untreated control. NTON at 150 μg mL−1 was the most potent to reduce the motility within 4 h whereas TCBZ and OCZN were much delayed. Histopathological changes showed swollen, extensive cracking, numerous vacuole...
Journal of the Bangladesh Agricultural University, 2013
To identify tick-borne pathogens in blood samples from 68 dogs were examined for Babesia, Anaplas... more To identify tick-borne pathogens in blood samples from 68 dogs were examined for Babesia, Anaplasma/Ehrichia, Mycoplasma, and Hepatozoon spp. by using a molecular tool. The detection of DNA was performed using specific primers for partial 16S rRNA or 18S rRNA sequence, and amplicon sequences were analyzed. Our investigation detected Babesia gibsoni (26 dogs) and Anaplasma sp. AnHl446 (2 dogs), Mycoplasma haemocanis (27 dogs), and an unknown Mycoplasma spp were detected (2 dogs), which was most closely related to Candidatus Mycoplasma turicensis, on the basis of phylogenetic analysis. Hepatozoon DNA could not be detected in this study.DOI: http://dx.doi.org/10.3329/jbau.v10i2.14915 J. Bangladesh Agril. Univ. 10(2): 249-253, 2012
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Papers by Hasanuzzaman Talukder BAU