Application of optical coherence tomography to non-destructively characterise rind breakdown disorder of ‘Nules Clementine’ mandarins
Postharvest Biology and Technology, Oct 1, 2013
ABSTRACT The feasibility of optical coherence tomography (OCT) for imaging histological changes a... more ABSTRACT The feasibility of optical coherence tomography (OCT) for imaging histological changes associated with the development of a progressive rind breakdown (RBD) disorder of ‘Nules Clementine’ mandarin (Citrus reticulate Blanco.) was investigated. The investigation utilised fruit with different levels of the disorder, carefully selected from a batch of fruit stored for eight weeks at ±8°C. Images of healthy and RBD-affected intact mandarin fruit were acquired using a Thorlabs OCT system based on a broadband 930 nm source. OCT provided high resolution 2D images of fruit rind to a depth of about 1.1 mm. Immediate and non-destructive acquisition of images showing histological and microstructural features of intact rind tissues was demonstrated. The oil glands stayed intact in unaffected fruit and gradually collapsed on RBD affected fruit. At advanced stages of the disorder, the collapsed oil glands became increasingly deformed and flattened. The study showed that OCT is a promising technique for immediate, real-time and non-destructive acquisition of images showing histological and microstructural rind features of ‘Nules Clementine’ mandarin fruit.
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Papers by Ralph Tatam