Establishment of 6 novel patient-derived sarcoma cell lines and 1 patient-derived carcinosarcoma cell line
Clinicopathologic Features of Non-CNS Primary Ewing Sarcoma Family of Tumors in the Head and Neck Region. Don’t Forget the CIC- and BCOR-rearranged Sarcomas
To the Editor: I have read, with much interest, the paper of Woo et al 1 on “Clinicopathologic fe... more To the Editor: I have read, with much interest, the paper of Woo et al 1 on “Clinicopathologic features of tumors in the head and neck region.” Nevertheless, I would like to make a comment concerning the 2 cases in their study with Ewing sarcoma family of tumors morphology, in which no EWSR1 gene rearrangement could be detected by reverse transcriptase-polymerase chain reaction and fluorescence in situ hybridization. Like already stated by the authors, an explanation in these cases could be that fluorescence in situ hybridization for EWSR1 might fail to detect rare Ewing sarcoma cases wherein FUS may displace EWSR1 (Ewing sarcoma with FUS rearrangement). However, “EWSR1-negative” small round cell sarcomas with Ewing sarcomalike morphology (Ewing-like sarcomas) are very heterogenous from molecular pathologic point of view and recent studies have revealed that some groups of Ewing-like sarcoma show typical Ewing sarcoma morphology but lack any EWSR1 of FUS rearrangements. A small number of these EWSR1/FUS negative cases sharing the undifferentiated Ewing sarcoma family of tumors appearance have been characterized recently carrying BCOR rearrangements (eg, BCOR-CCNB3, BCOR-MAML3, BCOR-ZC3H7B fusions) or CIC rearrangements (eg, CIC-DUX4, CIC-FOXO4 fusions), which could be an alternative explanation/differential diagnosis for the EWSR1-negative cases in this study. 2–5 Moreover, the head and neck region is one of the more common locations for CIC-rearranged sarcomas and recent findings of Owosho et al 6 suggest that head and neck CIC-rearranged sarcomas have different clinical presentation and pathologic features compared with Ewing sarcoma. Diagnosing these BCOR-rearranged or CIC-rearranged sarcomas can be difficult owing to their morphologic, immunohistochemical, and clinical overlap with Ewing sarcoma, especially with atypical Ewing sarcoma and tumors with unusual immunoprofiles or uncommon clinicoradiologic findings. Immunohistochemistry (ERG, WT1, ETV4, DUX4 immunohistochemical stainings in CIC-rearranged sarcomas; CCNB3 and BCOR immunohistochemical stainings in BCOR-rearranged sarcomas) could be of additional diagnostic value 7–9 and molecular testing is generally required to make a definite diagnosis in such cases.
Highly stable, pure and anhydrous organometallic YBa2Cu3O7-δ (YBCO) precursor solutions were prep... more Highly stable, pure and anhydrous organometallic YBa2Cu3O7-δ (YBCO) precursor solutions were prepared by dissolving commercial YBCO powder in acetone by trifluoroacetic anhydride (TFAA) or a mixture of TFAA with propionic acid for low fluorine precursors. The reaction is 72 times faster via microwave heating, compared to conventional oil bath heating. More importantly, the formation of by-products is suppressed, as shown by Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS). This approach allows a highly reproducible preparation of superconducting coatings which is of interest for a low-cost manufacturing processes capable of large-scale production of the coated conductors via Chemical Solution Deposition (CSD). This technology requires reliable and stable precursor solutions for continuous deposition. In this work, we obtained YBCO thin films on single-crystal substrates ((100)-LaAlO3) with a high critical current density (Jc) of 3-4 MA/cm² in self-field at 77 K using TFA-based YBCO precursors and Jc of 5-6 MA/cm² using low fluorine YBCO precursors.
Ectopic hamartomatous thymoma is a rare benign tumor in adults, mostly located in the lower neck ... more Ectopic hamartomatous thymoma is a rare benign tumor in adults, mostly located in the lower neck region. It was first reported in 1982 by Smith and McClure. Histopathologically these tumors are typically well marginated and composed of a mixture of spindle cells, mature adipose tissue, and epithelial cells, including both glandular and squamous elements. The histogenesis of this tumor is controversial. Recently, an origin from a remnant of the cervical sinus of His was proposed. Ectopic hamartomatous thymoma needs to be differentiated from malignant lesions such as synovial sarcomas or malignant peripheral nerve sheath tumors. These tumors can have similar clinical features and radiologic images. Recognition of this tumor is important because it follows a benign clinical course and conservative surgical excision is the treatment of choice. To our knowledge, 61 cases have previously been reported. We present the case of a 45-year-old women with an uncomplicated presternal located ectopic hamartomatous thymoma. The morphological and immunohistochemical findings are discussed and a review of literature is made.
Solitary fibrous tumour of the nasal cavity: a case report and literature review
PubMed, 2012
A solitary fibrous tumour (SFT) is a rare tumour that originates from the mesenchyme and arises m... more A solitary fibrous tumour (SFT) is a rare tumour that originates from the mesenchyme and arises mainly in the pleura. In this report, we present a rare case of a 77-year-old man with a SFT in the left nasal cavity. On CT and MRI, a large mass is seen in the left nasal cavity, extending to the choana. The mass was radically resected via functional endoscopic sinus surgery (FESS). Histological examination showed a fibroblastic mesenchymal tumour with a prominent hemangiopericytic vascular branching pattern that stained diffusely positive for the immunohistochemical markers CD34, Bcl-2, and CD99, which was compatible with the histopathological diagnosis of a SFT. Imaging and histological features are discussed, along with the reports in literature, clinical management, and follow-up of this pathology.
A Sclerosing Perineurioma With Collagen Rosette Formation: Benign Mimic of Low-Grade Fibromyxoid Sarcoma
International Journal of Surgical Pathology, Nov 26, 2017
We report the case of a sclerosing perineurioma with conspicious collagen rosette formation in a ... more We report the case of a sclerosing perineurioma with conspicious collagen rosette formation in a 20-year-old male presenting with a firm, painless nodule on the palmar side of his right ring finger. The main differential diagnosis is a low-grade fibromyxoid sarcoma. The distinction between these entities is important because low-grade fibromyxoid sarcoma has a metastatic potential, while, as a rule, perineuriomas are benign. The presence of collagen rosettes in this current case makes this distinction even more difficult given that approximately 30% of low-grade fibromyxoid sarcoma cases show the focal presence of collagen rosettes. The demonstration of the characteristic t(7;16), t(11;16) or t(11;22) translocations (resulting in the FUS-CREB3L2, FUS-CREB3L1 or EWSR1-CREB3L1 fusion genes, respectively) or immunoreactivity for MUC4, a recently described sensitive and specific marker for low-grade fibromyxoid sarcoma, remain the gold standard in the diagnosis of low-grade fibromyxoid sarcoma, differentiating it from perineurioma. This case is, to our knowledge, the first report on collagen rosettes in sclerosing perineurioma, extremely well mimicking low-grade fibromyxoid sarcoma, and further expanding the morphological spectrum of this rare subtype of perineurioma.
4 no no no atypical spindle cells yes no possible yes variable no no no lipoblasts common rare po... more 4 no no no atypical spindle cells yes no possible yes variable no no no lipoblasts common rare possible (not required) yes yes (pleomorphic) no no no pleomorphic MN cells yes no possible possible yes no no no mitoses often no possible variable high no variable variable necrosis no no no possible common no no no other characteristics MN floret cells + ropey like collagen prominent thick-walled vessels patternless growth and branching vessels Immunohistochemistry MDM2 and CDK4 positivity no no yes yes no no no no Rb loss yes (50-70%) yes no no yes yes yes no others desmin+ (diffuse, 90%) CD34+ (diffuse, 90%) ER+ (over 90%) PR+ (over 90%) STAT6+ Molecular features MDM2 or CDK4 amplification no no yes (close to 100%) yes (close to 100%) no no no no RB1 deletion yes yes no no yes yes yes no
Fibrohistiocytic tumors are among the most frequent soft tissue tumors encountered in the skin • ... more Fibrohistiocytic tumors are among the most frequent soft tissue tumors encountered in the skin • "Fibrohistiocytic" is an entirely descriptive term devised to designate a group of heterogeneous tumors resembling both normal fibroblasts and histiocytes • Unlike most similar designations (adipocytic, vascular,…), it does not cover a definitive line of differentiation • Most tumors in this category are of 'uncertain histogenesis' • Based on their histological features, cutaneous fibrohistiocytic tumors fall into benign, intermediate and malignant subcategories Benign fibrohistiocytic tumors • Benign fibrous histiocytoma & variants -cellular fibrous histiocytoma -aneurysmal fibrous histiocytoma -epithelioid fibrous histiocytoma -atypical fibrous histiocytoma • Cellular neurothekeoma
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