Papers by Prince Eric Perez
Gut Microbes, 2020
Sleep is a fundamental biological process, that when repeatedly disrupted, can result in severe h... more Sleep is a fundamental biological process, that when repeatedly disrupted, can result in severe health consequences. Recent studies suggest that both sleep fragmentation (SF) and dysbiosis of the gut microbiome can lead to metabolic disorders, though the underlying mechanisms are largely unclear. To better understand the consequences of SF, we investigated the effects of acute (6 days) and chronic (6 weeks) SF on rats by examining taxonomic profiles of microbiota in the distal ileum, cecum and proximal colon, as well as assessing structural and functional integrity of the gastrointestinal barrier. We further assayed the impact of SF on a host function by evaluating inflammation and immune response. Both acute and chronic SF induced microbial dysbiosis, more dramatically in the distal ileum (compared to other two regions studied), as noted by significant perturbations in alpha-and beta-diversity; though, specific microbial populations were significantly altered throughout each of the three regions. Furthermore, chronic SF resulted in increased crypt depth in the distal ileum and an increase in the number of villi lining both the cecum and proximal colon. Additional changes were noted with chronic SF, including: decreased microbial adhesion and penetration in the distal ileum and cecum, elevation in serum levels of the cytokine KC/GRO, and depressed levels of corticotropin. Importantly, our data show that perturbations to microbial ecology and intestinal morphology intensify in response to prolonged SF and these changes are habitat specific. Together, these results reveal consequences to gut microbiota homeostasis and host response following acute and chronic SF in rats.
The Relevance of the In Situ Mucin Adsorption on Solid Surfaces in Dentistry and Ophthalmology
Polymers in Medicine II, 1986
Mucosal surfaces in contact with the external biological environment are generally covered with a... more Mucosal surfaces in contact with the external biological environment are generally covered with a mucus layer. The primary function of this layer in the oral cavity and in the respiratory, gastrointestinal and reproductive tracts is to lubricate ephithelial cells and protect them from attack by microorganisms, the toxins they produce, and other antigens1–3.
The clinical role of granisetron (Kytril) in the prevention of chemotherapy-induced emesis
Seminars in oncology, 1994
Recent Advances in Fish Farms, 2011
fish meal and fish oils. Indeed, the aquaculture sector's consumption of fish meal and oils incre... more fish meal and fish oils. Indeed, the aquaculture sector's consumption of fish meal and oils increased respectively from 2.9Mt to 3.7Mt and from 0.6Mt to 0.8Mt between 2000 and 2008 (Tacon and Metian, 2008). Over and above the issue relating to the use of feed with a high biological value for aquaculture production, Naylor et al. (2000) contrast two aquaculture models: the first one, an input-intensive system, in particular as regards fish meal and oils and a priori non sustainable, and the second one, classically described as extensive or semi-extensive, considered to be sustainable. This implicit or explicit assimilation of intensive and extensive/semi-extensive systems with models of respectively weak or strong sustainability can be found in many publications from the 1980s and 1990s (
The Surface Force Apparatus (SFA) measures directly, and with nanoscale resolution, the interacti... more The Surface Force Apparatus (SFA) measures directly, and with nanoscale resolution, the interaction energy vs. distance profile of planar arrays of biological molecules (e.g., lipids, polymers, or proteins). Through recent advances in the reconstitution and deposition of lipid bilayers, it is now possible to use SFA to study the interactions between membrane-incorporated biomolecules and to reveal any conformational changes and intermediate assembly states. Therein we describe two example systems. First, we show that using bilayers functionalized to carry DNA bases on their lipid headgroups, we can measure a macroscopic nucleosidenucleoside adhesion force, from which one can obtain a molecular binding energy. Second, we describe the use of the SFA to study the interaction between SNARE proteins, which are involved in most of intracellular fusion events. Membrane fusion occurs when SNARE proteins assemble between lipid bilayers in the form of SNAREpins. SFA measurements between SNAREs embedded in lipid bilayers allowed us to elucidate the energetics and dynamics of SNAREpin folding, and to capture an intermediate binding state in SNAREpin assembly.
Langmuir, 1995
To help understand the effects of protein adsorption on membrane filtration performance, we have ... more To help understand the effects of protein adsorption on membrane filtration performance, we have measured the molecular interactions between cellulose acetate films and two proteins with different properties (ribonuclease A and human serum albumin) with a surface force apparatus. Comparison of forces between two protein layers with those between a protein layer and a cellulose acetate (CAI film shows that, at high pH, both proteins retained their native conformation on interacting with the CA film while at the isoelectric point (PI) or below the tertiary structure of proteins was disturbed. These measurements provide the first molecular evidence that disruption of protein tertiary structure could be responsible for the reduced permeation flows observed during membrane filtration of protein solutions and suggest that operating at high pH values away from the PI of proteins will reduce such fouling.
Ultra-Compact Long-Period Fiber Grating and Grating Pair Fabrication using a Modulation-Scanned CO2 Laser
OFC/NFOEC 2007 - 2007 Conference on Optical Fiber Communication and the National Fiber Optic Engineers Conference, 2007
Page 1. Ultra-Compact Long-Period Fiber Grating and Grating Pair Fabrication using a Modulation-S... more 
Uploads
Papers by Prince Eric Perez