Papers by J. ISABEL LÓPEZ-ARROYO
Insecticidas Efectivos para el Control de Ninfas del Psílido Asiático de los Cítricos1 en Limón Persa
Southwestern Entomologist
Citrus crops represent an outstanding activity at worldwide level. Mexico ranks fourth place in p... more Citrus crops represent an outstanding activity at worldwide level. Mexico ranks fourth place in production. Due introduction and dispersion of D. citri vector in all citric zones from Mexico and the newly detected disease known as huanglongbing (HLB) caused by Candidatus Liberibacter asiaticus bacteria, assessment of control methods for this bug is required. In 2010, technical effectiveness in field and persistence for different action route against Persian lime D. citri chemical products tests were performed in Nayarit, Mexico. Two assessments were made at different dates. The amount of nymphs per shoot and percentage of infested shoots were assessed in each rehearsal. From evaluated products, dimethoate, imidacloprid and the mixture of imidacloprid and β-cyfluthrin exerted more than 85% percent of control against nymphs during 27 days after application. In trees treated with dimethoate and imidacloprid in 400 mL and 300 mL ha-1 dose, respectively, 100% of shoots free of D. citri nymphs at 27 days after application was observed. Agricultural oil in two and three L ha-1 dose exerted less control against D. citri nymphs.
El impacto económico y social estimado del HLB, a tres años de establecido, en un escenario de al... more El impacto económico y social estimado del HLB, a tres años de establecido, en un escenario de alta presencia en México, sería la pérdida de 1.7 millones de toneladas y of Rosario, Sonora, and Pichucalco, Chiapas, presented 34 and 28 potential generations per year respectively. The maximum risk, IRPM, was found in the municipalities of
The aim of this study was to estimate yield losses induced by the agent associated with Huanglong... more The aim of this study was to estimate yield losses induced by the agent associated with Huanglongbing, Candidatus Liberibacter asiaticus (CLas) in Persian lime (Citrus latifolia Tanaka) in Mexico, by evaluating morphological and organoleptic fruit variables. A four-year old Persian lime orchard in the state of Yucatán, Mexico, was selected; the trees were under same disease management and infection; this feature was verified by qPCR. The study was conducted under four severity levels (0=healthy, 1=25, 2=50, 3=75, 4=100, percentage of canopy with HLB symptoms). Eight morphological and organoleptic variables were evaluated in a restricted random block design with healthy and CLas infected trees. The values of weight, size, skin thickness, juice volume (JV) and pH were statistically higher in fruits from healthy trees as well as in asymptomatic branches of positive trees, in comparison with symptomatic branches (Tukey, P=0.05). CLas induced reduction on weight (17.3 %) and JV (18.6 %), with more damage in trees showing 100 % of canopy with HLB symptoms; the regression models were: Y weight = 217.2-4.2x+0.03x 2 , R 2 = 0.86; Y volume = 645.4-11.7x+0.09x 2 , R 2 = 0.82.
Current situation and prospects of management of citrus HLB
Toxicity of methyl bromide as a postharvest treatment on mangoes and stone fruit against the Mexican fruit fly and the West Indian fruit fly Diptera: Tephritidae 131
Tropical Agriculture, 2016
First and second instar Mexican fruit fly, Anastrepha ludens (Loew), larvae were more resistant t... more First and second instar Mexican fruit fly, Anastrepha ludens (Loew), larvae were more resistant to methyl bromide (MB) than 1, 2, and 3 day old eggs and third instar larvae. Concentrations of 55 and 60 gMB/m3 delivered for 2 h caused 99.9968% mortality of larvae of the Mexican fruit fly and West Indian fruit fly, A. obliqua (Loew), respectively, in mangoes Mangifera indica L. Doses of MB were statistically equal and caused 99.9968% mortality of both larvae and pupae of A. ludens and A. obliqua. At 48 g MB/m3 for 3.3h 99.9968% mortality of Mexican fruit fly larvae was determined. Concentrations of 46 g/m3 were estimated to cause 99.9968% mortality of Mexican fruit fly (based on estimated larvae and larval mortality) in peaches, Prunnis persicae (L.) Batsch, plums, P. salicina Lindl. and nectarines, P. persicae (L.) Batsch nectrina. Temperatures in the climatic chamber at the time of treatment ranged from 13.0°C to 34.6°C and did not reduce efficacy of this fumigant against either fly...
Journal of Citrus Pathology, 2014
Journal of Citrus Pathology, 2014
Journal of Citrus Pathology, 2014
American Entomologist, 2017
He had high standards in everything he did, was rigorous, and demanded much from himself and othe... more He had high standards in everything he did, was rigorous, and demanded much from himself and others. His oft-repeated mantra "Work hard, play fair, live clean!" was the target of good-natured banter over the years, but it truly exemplified his approach to life. He was both passionate and compassionate, and his interest in and dedication to his students endured not only through graduate school, but for life. He had a keen sense of humor and an impressive intellect. He was perhaps the only person in our lives who could readily quote Socrates, Shakespeare, and Darwin. Now, more than three years after his death, Professor Tauber's voice and visage are as clear in our memories as when we last saw him-a testament to the indelible impact he had on us.
Revista Mexicana de Ciencias Agrícolas, 2018
La efectividad del insecticida ometoato (Folimat® 1000) y de enemigos naturales nativos para cont... more La efectividad del insecticida ometoato (Folimat® 1000) y de enemigos naturales nativos para controlar al psílido asiático de los cítricos, fue comparada en Río Bravo, Tamaulipas, durante 2010. Los resultados indicaron que ometoato fue más efectivo y no se observaron ninfas o adultos a las 264 h después de la aplicación; por otra parte, a las 72 h en el tratamiento de enemigos naturales, la población de ninfas fue reducida y similar al tratamiento con ometoato. En el testigo absoluto, la población de ninfas se incrementó a más 100% a las 264 h.
Southwestern Entomologist, 2016
Resumen. El presente estudio se realizó con el objetivo de identificar las especies de crisopas (... more Resumen. El presente estudio se realizó con el objetivo de identificar las especies de crisopas (Neuroptera: Chrysopidae) presentes en parcelas de sorgo infestadas con el pulgón del sorgo Melanaphis sacchari (Zehntner) (Hemiptera: Aphididae), en el norte de Sinaloa, México. De 424 individuos colectados se determinaron 416, obteniendo ocho especies: 1. Ceraeochrysa caligata (Banks), 2. Ceraeochrysa cubana (Hagen), 3. Ceraeochrysa sp. nr. cincta (Schneider), 4. Ceraeochrysa valida (Banks), 5. Chrysoperla carnea s. lat. (Stephens), 6. Chrysoperla externa (Hagen), 7. Chrysoperla comanche (Banks), y 8. Chrysoperla rufilabris (Burmeister). Chrysoperla externa registró la mayor cantidad, seguida por C. carnea, y en tercer sitio de abundancia se ubicó C. cubana. Las crisopas cargabasura C. caligata, C. cubana, y C. sp. nr. cincta, son registradas por primera ocasión para Sinaloa. Por su abundancia y frecuente presencia en colonias de M. sacchari, se sugiere la cría masiva de C. cubana para su eventual uso en el control biológico de la plaga.
African Journal of Biotechnology, Oct 30, 2012
Hirsutella sp. was grown in four liquid media containing either casamino acids, corn steep liquor... more Hirsutella sp. was grown in four liquid media containing either casamino acids, corn steep liquor, collagen peptone or casein peptone. These media were inoculated with a 7 day-old culture of mycelia and blastospores of Hirsutella sp. and the cultures incubated with shaking at 250 rpm at 26°C. The media containing corn steep liquor, casamino acids or collagen peptone produced abundant mycelium, varying from 64 to 76.3 mg/ml at different fermentation days, whereas the medium containing casein peptone produced less biomass. Additionally, the casamino acids and collagen peptone media showed similar biomass values, as well as blastospore counts after 14 fermentation days, without significant differences between the two media. The highest number of blastospores produced was 3.8 × 10 7 blastospores/ml using medium with casamino acids. This medium, as well as that with collagen peptone gave suitable results for the preparation of an inoculum for the production of conidia in solid medium.
Journal of Entomological Science, 2015
BIOtecnia, 2012
La enfermedad Huanglongbing (HLB) de los cítricos aun no ha sido diagnosticada en Sonora. Actualm... more La enfermedad Huanglongbing (HLB) de los cítricos aun no ha sido diagnosticada en Sonora. Actualmente se lleva a cabo un plan regional para el manejo del psílido asiático de los cítricos, Diaphorina citri. En áreas citrícolas con producción orgánica, el uso de insecticidas sintéticos se encuentra contemplado solo si la enfermedad del HLB es diagnosticada en ellas. Es por ello, que se planteó la búsqueda de productos alternativos efi caces para uso orgánico con diferentes mecanismos de acción. En árboles de naranja valencia en desarrollo se probaron 8 productos orgánicos, Acceem ® , Cassia ® , Java ® , Knockout ® , Mosei ® y Oroboost ® (1 mL/L), Gardytec ® +Agrosiamil ® (1,5:1,5 mL/L), PureSpray ® (2,5 mL/L) y un control, Muralla Max ® (0,25 mL/L). Aplicados por aspersión sobre ninfas y adultos de D. citri, con muestreos a los 5 y 10 días después de la aplicación (DDA). Los mejores resultados se observaron a los 5 DDA, con una efi cacia de 98,45%, 78,93% y 74,52%, para Oroboost, Mosei y Agro-siamil+Gardytec respectivamente. Los productos probados funcionaron solamente sobre estadios ninfales.
Genetic variability analysis of entomopathogenic fungi isolated from citrus-growing areas of Mexico
Microorganisms in Industry and Environment - From Scientific and Industrial Research to Consumer Products - Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009), 2010
The objective of the study was to isolate and characterize genetically native fungi that remain i... more The objective of the study was to isolate and characterize genetically native fungi that remain in the soil of the diverse citrus regions of Mexico. We collected 142 soil samples from different Mexican states: Campeche (13), Michoacan (15), Nuevo Leon (25), San Luis Potosi (25), Sinaloa (17), Sonora (17), Tabasco (5), Tamaulipas (20), and Yucatan (5). Larvae of Galleria mellonella were used as bait to detect, trap and multiply entomopathogenic fungi in vivo. 23% of the soil samples processed were positive for the presence of entomopathogenic fungi according to the macroscopic and microscopic characteristics: Beauveria was detected in 12% (17 isolates), Metarhizium in 1% (2 isolates) and Isaria in 10% (14 isolates). We analyzed the genetic variability of the fungi by using the sequences of internal transcribed ribosomal genes (ITS-5.8S-ITS2) and subsequently performed the digestion with the enzyme Hae III. The results of the PCR generated fragments of approximately 600 bp for Beauveria spp., and Metarhizium spp. In the case of Isaria spp., it amplified a fragment above of 600 bp. Both reference strains and field isolates produced similar restriction patterns. Genera of native entomopathogenic fungi were found on the diverse citrus areas of Mexico; this opens the field for the biological control of pests affecting citrus in each region.
Plant Disease, 2017
Huanglongbing (HLB), a recent worldwide spreading disease on citrus, was detected in July 2009 in... more Huanglongbing (HLB), a recent worldwide spreading disease on citrus, was detected in July 2009 in Yucatan State of Mexico. The objective of this study was to evaluate the fit of diffusion and classic disease gradient models to large-scale HLB spatial data originated from initial foci to improve sampling, monitoring, and control strategies for Diaphorina citri, vector of Candidatus Liberibacter asiaticus (CLas), putative agent of HLB. Four transect routes were selected: Yuc-1, Yuc-2, QRoo-1, and QRoo-2, based on the directionality of the prevailing winds and foci location of HLB infected plants. In these routes, 35 sites, 5 to 20 km apart, were selected for monthly evaluation during a 12-month period. A 10-insect sample and disease incidence and severity of HLB, further confirmed by PCR, were assessed per site. Mexican lime was more vulnerable (67.5%) than sweet orange (14%). Also, leaf symptoms were mostly found with homogeneous distribution but rarely reaching 100% of the tree cano...
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Papers by J. ISABEL LÓPEZ-ARROYO