Papers by Jirundon Yuvaniyama
BRCA1 deficiency enhances the aggressiveness of breast cancer cells expressing HPV16 oncoproteins
Biology of the cell, Mar 21, 2024
Acta Crystallographica Section D-biological Crystallography, Mar 23, 2004
Double mutant Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS-K1, C59R+S108N) complexed with Trimethoprim (TOP), NADPH and dUMP
Human VH1-RELATED Dual-Specificity Phosphatase
Wild-type Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS) complexed with methotrexate (MTX), NADPH and dUMP
The structure of the orthorhombic form of soft-shelled turtle lysozyme at 1.9 angstroms resolution
Acta crystallographica, Feb 24, 2005
Methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase (MHPCO) catalyzes the conversion of a... more Methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase (MHPCO) catalyzes the conversion of an aromatic substrate, MHPC, to an aliphatic compound,-(N-acetylaminomethylene)-succinic acid, and is involved in the degradation of vitamin B 6 by the soil bacterium Pseudomonas sp. MA-1. Using only FAD as a cofactor, MHPCO is unique in catalyzing hydroxylation and subsequent aromatic ring cleavage without requiring a metal-ion cofactor. Here, the crystallization of MHPCO is reported together with preliminary X-ray crystallographic data. An MHPCO crystal obtained by hanging-drop vapour diffusion diffracted X-rays to 2.25 A Ê resolution and belonged to the triclinic space group P1, with four molecules per asymmetric unit. crystallization communications 314 Oonanant et al. MHPC oxygenase Acta Cryst. (2005). F61, 312±314 Figure 3 A 1-rotation photograph showing the X-ray diffraction pattern of an MHPCO crystal. The arrow indicates a re¯ection of 2.3 A Ê resolution.
Acta crystallographica, Feb 28, 2006
An engineered mutant V107A of the dimeric glutathione transferase enzyme from Anopheles dirus (ad... more An engineered mutant V107A of the dimeric glutathione transferase enzyme from Anopheles dirus (adgstD4-4) was cocrystallized with glutathione substrate using the hanging-drop vapour-diffusion method. The crystal diffracted to 2.47 Ã… resolution in space group P3 2 21 (unit-cell parameters a = b = 49.4, c = 272.4 Ã…). Although the crystal morphology differed from that previously obtained for the wild-type enzyme, the crystal packing was the same. At 318 K, the engineered mutant showed an enzyme stability that was increased by about 32-fold, while possessing a similar catalytic function to the wild type. Structural determination will provide valuable understanding of the role of Val107. This residue is in the dimeric interface and appears to contribute towards enhancing the physical properties of the entire protein.
Acta crystallographica, Oct 30, 2009
Trypanosoma cruzi dihydrofolate reductase-thymidylate synthase (TcDHFR-TS) was crystallized in co... more Trypanosoma cruzi dihydrofolate reductase-thymidylate synthase (TcDHFR-TS) was crystallized in complexes with the dihydrotriazine-based or quinazoline-based antifolates C-448, cycloguanil (CYC) and Q-8 in order to gain insight into the interactions of this DHFR enzyme with classical and novel inhibitors. The TcDHFR-TS-C-448-NDP-dUMP crystal belonged to space group C222 1 with two molecules per asymmetric unit and diffracted to 2.37 Ã… resolution. The TcDHFR-TS-CYC, TcDHFR-TS-CYC-NDP and TcDHFR-TS-Q-8-NDP crystals belonged to space group P2 1 with four molecules per asymmetric unit and diffracted to 2.1, 2.6 and 2.8 Ã… resolution, respectively. Crystals belonging to the two different space groups were suitable for structure determination.
Crystal structure of the reductase (C1) component of p-hydroxyphenylacetate 3-hydroxylase (HPAH) from Acinetobacter baumannii
Acta crystallographica, Sep 13, 2005
Chitinase A of Vibrio carchariae was expressed in Escherichia coli M15 host cells as a 575-amino-... more Chitinase A of Vibrio carchariae was expressed in Escherichia coli M15 host cells as a 575-amino-acid fragment with full enzymatic activity using the pQE60 expression vector. The yield of the highly purified recombinant protein was approximately 70 mg per litre of bacterial culture. The molecular mass of the expressed protein was determined by HPLC/ESI-MS to be 63 770, including the hexahistidine tag. Crystals of recombinant chitinase A were grown to a suitable size for X-ray structure analysis in a precipitant containing 10%(v/v) PEG 400, 0.1 M sodium acetate pH 4.6 and 0.125 M CaCl 2. The crystals belonged to the tetragonal space group P422, with two molecules per asymmetric unit and unitcell parameters a = b = 127.64, c = 171.42 Ã…. A complete diffraction data set was collected to 2.14 Ã… resolution using a Rigaku/MSC R-AXIS IV ++ detector system mounted on an RU-H3R rotating-anode X-ray generator.
Genome Biology and Evolution
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread globally, and sc... more Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread globally, and scientists around the world are currently studying the virus intensively in order to fight against the on-going pandemic of the virus. To do so, SARS-CoV-2 is typically grown in the lab to generate viral stocks for various kinds of experimental investigations. However, accumulating evidence suggests that such viruses often undergo cell culture adaptation. Here, we systematically explored cell culture adaptation of two SARS-CoV-2 variants, namely the B.1.36.16 variant and the AY.30 variant, a sub lineage of the B.1.617.2 (Delta) variant, propagated in three different cell lines, including Vero E6, Vero E6/TMPRSS2, and Calu-3 cells. Our analyses detected numerous potential cell culture adaptation changes scattering across the entire virus genome, many of which could be found in naturally circulating isolates. Notable ones included mutations around the spike glycoprotein's multibasic clea...
Rice BGlu1 beta-glucosidase, a plant exoglucanase/beta-glucosidase
The structure of the orthorhombic form of soft-shelled turtle lysozyme at 1.9 angstroms resolution
WILD TYPE PLASMODIUM FALCIPARUM DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASE (PfDHFR-TS), DHF COMPLEX, NADP+, dUMP
Double mutant (A16V+S108T) Plasmodium falciparum DHFR-TS (T9/94) complexed with cycloguanil, NADPH and dUMP
Autoprocessing abilities of semi-pro-PM-I samples during a prolonged dialysis
<p>Autoactivation abilities of the samples from the same dialysis experiment as shown in &l... more <p>Autoactivation abilities of the samples from the same dialysis experiment as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089424#pone-0089424-g004" target="_blank">Figure 4</a> at 0, 6, 9, 24, and 48 h were evaluated for protein band shift in SDS-PAGE from around 45-kDa unactivated samples (U) to 37-kDa mature PM-I (A).</p
Immunogenicity of snake α-neurotoxins and the CD4 T cell epitopes
Toxicon
Purification and characterization of amine oxidase from
This article appeared in a journal published by Elsevier. The attached copy is furnished to the a... more This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier’s archiving and manuscript policies are encouraged to visit:
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Papers by Jirundon Yuvaniyama