Background Acinetobacter baumannii is a major opportunistic pathogen in hospital-acquired infecti... more Background Acinetobacter baumannii is a major opportunistic pathogen in hospital-acquired infections, posing challenges for clinical control. This study aimed to isolate and characterize a novel strain of A. baumannii phage from hospital wastewater and to determine its biological properties and evaluate its in vitro and in vivo bacteriostatic effects. Methods Using A. baumannii NO.424 as the host bacterium, phages were isolated and purified using the plaques method and double-layer agar method. The host spectrum and phage microscopic morphology were determined using the plaques method and transmission electron microscopy, respectively. The Optimal multiplicity of infection, adsorption curve, one-step growth curve, thermal stability, acid–base stability, UV tolerance, and chloroform stability were determined using the double-layer agar method. Bioinformatic functional analysis was performed after genome sequencing using the Illumina platform. The in vitro and in vivo inhibitory effec...
Characterization and antimicrobial activity of a novel lytic phage vB_SmaS_QH16 against Stenotrophomonas maltophilia: in vitro, in vivo, and biofilm studies
Frontiers in Cellular and Infection Microbiology, 2025
Background Stenotrophomonas maltophilia , an important opportunistic pathogen resistant to multip... more Background Stenotrophomonas maltophilia , an important opportunistic pathogen resistant to multiple antibiotics, necessitates alternative therapies. Phages, with their high specificity and bacteriolytic ability, are emerging as promising antibiotic alternatives. This study aimed to isolate and characterize a novel lytic phage targeting S. maltophilia and to evaluate its antibacterial potential. Methods A novel lytic phage, vB_SmaS_QH16, was isolated from hospital sewage using S. maltophilia no.981 as the host. Phage morphology was analyzed using transmission electron microscopy (TEM), and genome sequencing and annotation were performed. Host range, efficiency of lysis (EOP), optimal multiplicity of infection (MOI), one-step growth curves, and physicochemical stability were also determined. Biofilm inhibition and eradication were assessed using crystal violet staining, MTT assays, and acridine orange fluorescence microscopy. Using Galleria mellonella and mouse infection models, the i...
Characterization of the novel cross-genus phage vB_SmaS_QH3 and evaluation of its antibacterial efficacy against Stenotrophomonas maltophilia
Frontiers in Microbiology, 2025
Background Bacteriophages, which are natural bacterial predators, demonstrate potential as safe a... more Background Bacteriophages, which are natural bacterial predators, demonstrate potential as safe and effective biological control agents against drug-resistant infections. This study aims to characterize the biological properties of the novel lytic phage vB_SmaS_QH3 and comprehensively evaluate its efficacy in preventing and controlling clinically multidrug resistance Stenotrophomonas maltophilia infections using both in vivo and in vitro models. Methods The phage was isolated from hospital sewage using the multidrug resistant S. maltophilia no. 3738 as the host. Transmission electron microscopy (TEM) was used to observe phage morphology, and the host range was determined via spot assays. Proliferation kinetics, including multiplicity of infection (MOI), adsorption rate, and one-step growth curves, were analyzed. Stability was assessed under various physicochemical conditions. Based on Illumina whole-genome sequencing data, bioinformatics tools were employed for gene annotation, func...
Effect of Biodegradable Microplastics and Cd Co-Pollution on Cd Bioavailability and Plastisphere in Soil-Plant System
Contamination characteristics and dietary intake risk of brominated flame retardants in fishes around a typical e-waste dismantling site in Southern China
Science of the total environment, Jun 1, 2024
Middle to Late Holocene lake level variations recorded by shoreline tufa of Longmu Co Lake, northwestern Tibet Plateau
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An Approach for Assessing Human Respiration CO<sub>2</sub> Emissions Using Radiocarbon Measurements and Bottom‐Up Data Sets
Journal of geophysical research. Atmospheres, Apr 29, 2024
The Measurement Reliability of δ13C of Dissolved Organic Carbon: A Validation for Speleothem Samples
Minerals, Apr 25, 2024
The carbon isotopic composition of dissolved organic carbon (δ13CDOC) in speleothems, based on hi... more The carbon isotopic composition of dissolved organic carbon (δ13CDOC) in speleothems, based on high-precision U-Th ages, has great potential to reconstruct past ecology, microbial activity, and carbon cycling. Identifying the reliability of the pretreatment and measurement of speleothem δ13CDOC is therefore essential to putting it into practice. Actually, we have previously verified the reliability of the method for speleothem δ13CDOC measurements. However, it was only based on the final δ13CDOC values of homogeneous speleothem powder and is unable to evaluate the effects of different experimental conditions and the impacts of adding acid on δ13CDOC if using speleothem as the study samples. In this study, we used an organic reagent (potassium sorbate) as the study sample and designed a conditional experiment that simulates the protocols for speleothem δ13CDOC analysis and presented the resulting data to inspect the effects of the experimental processes on the analysis of δ13CDOC. Th...
Impacts of Climate Change on Runoff in the Heihe River Basin, China
Atmosphere, Apr 23, 2024
Located in the central part of the arid regions of Northwest China, the Heihe River Basin (HRB) p... more Located in the central part of the arid regions of Northwest China, the Heihe River Basin (HRB) plays an important role in wind prevention, sand fixation, and soil and water conservation as the second largest inland river basin. In the context of the warming and wetting climate observed in Northwest China, the situation of the ecological environment in the HRB is of significant concern. Using the data from meteorological observation stations, grid fusion and hydrological monitoring, this study analyzes the multi-scale climate changes in the HRB and their impacts on runoff. In addition, predictive models for runoff in the upper and middle reaches were developed using machine learning methods. The results indicate that the climate in the HRB has experienced an overall warming and wetting trend over the past 60 years. At the same time, there are clear regional variabilities in the climate changes. Precipitation shows decreasing trends in the northwestern part of the HRB, while it shows...
A shift in the South China Sea marine radiocarbon reservoir age associated with the opening of the Balabac Strait
Palaeogeography, palaeoclimatology, palaeoecology, Jun 1, 2024
Enhanced hydrolysis and methane yield of temperature-phased dewatered sludge anaerobic digestion by microbial electrolysis cell
Bioresource technology, May 1, 2024
Supplemental materials
Figshare, 2021
Supplemental tables and figures
Monsoon-regulated marine carbon reservoir effect in the northern South China Sea
Zenodo (CERN European Organization for Nuclear Research), Sep 27, 2022
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