Papers by Pierre Sourdille
Biology
Improving the production of all crops is crucial to meeting the challenge of the growing needs re... more Improving the production of all crops is crucial to meeting the challenge of the growing needs related to the simultaneous increase in the world population and demands from farmers and end-users [...]
Generation of Deletion Lines in Allohexaploid Bread Wheat
Plant Gametogenesis
Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its l... more Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its large genome, high density of repeats, low recombination rate especially in pericentromeric regions and its allopolyploidy. One way to face this challenge is to decrease the size of the interval bearing the gene of interest both genetically and physically, in order to reduce significantly the number of potential candidate genes. In this chapter, we describe a technical approach to produce chromosome-specific deletion lines to locate precisely genes of interest onto wheat chromosomes, a step forward to their cloning.
The Plant Journal, 2018
Recombination affects the fate of alleles in populations by imposing constraints on the reshuffli... more Recombination affects the fate of alleles in populations by imposing constraints on the reshuffling of genetic information. Understanding the genetic basis of these constraints is critical for manipulating the recombination process to improve the resolution of genetic mapping, and reducing the negative effects of linkage drag and deleterious genetic load in breeding. Using sequence-based genotyping of a wheat nested association mapping (NAM) population of 2,100 recombinant inbred lines created by crossing 29 diverse lines, we mapped QTL affecting the distribution and frequency of 102 000 crossovers (CO). Genome-wide recombination rate variation was mostly defined by rare alleles with small effects together explaining up to 48.6% of variation. Most QTL were additive and showed predominantly transacting effects. The QTL affecting the proximal COs also acted additively without increasing the frequency of distal COs. We showed that the regions with decreased recombination carry more single nucleotide polymorphisms (SNPs) with possible deleterious effects than the regions with a high recombination rate. Therefore, our study offers insights into the genetic basis of recombination rate variation in wheat and its effect on the distribution of deleterious SNPs across the genome. The identified transacting additive QTL can be utilized to manipulate CO frequency and distribution in the large polyploid wheat genome opening the possibility to improve the efficiency of gene pyramiding and reducing the deleterious genetic load in the low-recombining pericentromeric regions of chromosomes.
Molecular biology and evolution, 2002
Ciblage de la recombinaison méiotique chez le blé tendre : Comment ça marche ?
Le Centre pour la Communication Scientifique Directe - HAL - CHU de Clermont-Ferrand, May 1, 2017
Ciblage de la recombinaison méiotique chez le blé tendre : Comment ça marche ?. Journée de l'... more Ciblage de la recombinaison méiotique chez le blé tendre : Comment ça marche ?. Journée de l'Ecole Doctorale SVSAE, les 20 an
Introduction de gènes de résistance à la rouille couronnée dans la sélection française d'avoine (... more Introduction de gènes de résistance à la rouille couronnée dans la sélection française d'avoine (Avena sativa L.
WheatPhenotypeOntology-v2.0a.txt
Wheat Phenotype ontology
Additional file 6: of Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome
IWGSC RefSeq v1.0 7A intra-scaffold gaps related to Gydle scaffolds and contigs plus bridging seq... more IWGSC RefSeq v1.0 7A intra-scaffold gaps related to Gydle scaffolds and contigs plus bridging sequences. (XLSX 398 kb)
Additional file 5: of Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome
a IWGSC RefSeq 7A inter-scaffold gaps related to PacBio and Gydle scaffolds. b Gydle island IDs r... more a IWGSC RefSeq 7A inter-scaffold gaps related to PacBio and Gydle scaffolds. b Gydle island IDs related to Bionano maps and BAC sets. (ZIP 51 kb)
Additional file 2: of Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome
Figures S1–S7. (with legends) for Additional files. (DOCX 5813 kb)
Additional file 12: of Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome
Rice chromosome 8 centromere synteny analysis with chromosome 7A. (DOCX 14 kb)
Additional file 10: of Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome
Finished 7A yield region assembly BAC sets. (XLSX 10 kb)
Exploitation of the 5BS Physical Map to Complete the SKr Crossability Locus
International audienc
Mapping key loci controlling the accumulation of micronutrients in kernel using a panel of interspecific introgression lines of T.urartu in T.monococcum
Theoretical and Applied Genetics, 2020
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Detection of QTL for bread-making quality in wheat using molecular markers
Developments in Plant Breeding, 1999
Bread-making quality in wheat (Triticum aestivum) is a complex trait influenced at once by geneti... more Bread-making quality in wheat (Triticum aestivum) is a complex trait influenced at once by genetic factors and environmental conditions. The development of molecular biology technics together with intervarietal genetic maps provide a useful tool for the understanding of complex agronomic traits such as quality. The aim of our study was to identify QTL for bread-making quality using a doubled-haploid lines population issued from a cross between Courtot and Chinese Spring. Three hundred and eighty loci were mapped using a subset of 106 lines and 187 lines were genotyped for the anchor markers of this map. Between 144 and 172 DH lines were grown under field conditions for three consecutive years (1994 and 1995: one replication; 1996 two replications) and between 106 and 163 were submitted to several technological tests. We were thus able to approach the genetic control of quality traits (strength (W), grain hardness and protein content) that were highly contrasted between the two parents. Despite the fact that the D genome was under represented because of a lack of polymorphism, it appeared that an important part of the variation was explained by the QTL located at different loci than those of the storage proteins (glutenins and gliadins), which seemed to play a minor role compared to other regions of the genome. We are now planning to study some of these regions to identify the genes that could be involved in the baking process.
Genomic DNA Isolation, Southern Blotting and Hybridization
Plant Molecular Biology — A Laboratory Manual, 1997
The isolation of plant nucleic acids is a fundamental requirement for most genome characterizatio... more The isolation of plant nucleic acids is a fundamental requirement for most genome characterization and mapping procedures involving the use of genetic markers, and for the identification and isolation of plant genes for genetic engineering. The degree of purity and quality required of the DNA isolated varies from application to application. On the one hand, high molecular weight, high purity DNA is required for the production of genomic DNA libraries, which are screened for plant gene sequences and for other genetic markers such as RFLPs. For genetic analysis, on the other hand, the degree of purity required may be lower, but other factors, such as the yield of DNA, may be more important.
Importance of diagnostic markers for the management of soil-borne viral diseases of barley and wheat
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Papers by Pierre Sourdille