Tonino Traini

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三次元印刷PLA足場とヒト歯肉幹細胞由来細胞外小胞:骨欠損修復のための新しいツール【JST・京大機械翻訳】

ナノコンポジットシステムChitlac‐nAgに対するヒト歯肉線維芽細胞/Streptococcus mitis共培養物の接着

Journal of Materials Science: Materials in Medicine, 2016

A Histologic and Histomorphometric Evaluation of Anorganic Bovine Bone Retrieved 9 Years After a Sinus Augmentation Procedure

Journal of Periodontology, May 1, 2007

ABSTRACT

Assessments of Prevalence of Ponticulus Posticus, Atlas Posterior Arch Deficiency, Sella Turcica Bridging, Maxillary Canine Impaction, and Associations Among Them in 500 CBCTs of Italian Orthodontic Patients

Frontiers in Dental Medicine, 2021

Background: The ponticulus posticus (PP) is an important anomaly of the atlas (C1 vertebra) with ... more Background: The ponticulus posticus (PP) is an important anomaly of the atlas (C1 vertebra) with a complete or partial bone bridge that transforms the groove of the vertebral artery (VA) into a canal (arcuate foramen). The aim was to retrospectively determine prevalence of PP evaluating morphological features and the possible association with other dentoskeletal anomalies in the midface and neck area such as maxillary canine impaction (MCI), atlas posterior arch deficiency (APAD), and sella turcica bridging (SB).Methods: This study was conducted in the Department of Medical, Oral and Biotechnological Sciences of the University “G. D'Annunzio” in Chieti. The detection of PP was performed on 500 patients with cone beam CT (CBCT) images (6 to 87 years).Results: Analysis revealed the presence of uni- and bi-lateral PP in 110 patients (22%): 24 (4.8%) patients with bilateral complete PP, 12 (2.4%) patients with complete PP on the left, 6 (1.2%) patients with complete PP on the right,...

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Surface microstructure and fibrine extension on titanium laser sintered specimens

Europerio 5, 2006

Additional file 3: of Three-dimensional printed PLA scaffold and human gingival stem cell-derived extracellular vesicles: a new tool for bone defect repair

Gene expression. (A) The expression value of genes for "regulation of ossification" and... more Gene expression. (A) The expression value of genes for "regulation of ossification" and "ossification" differentially expressed between 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGMSCs and compared with hGMSCs. (B) The expression value of genes for "regulation of osteoblast differentiation" and "osteoblast differentiation" differentially expressed between 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGMSCs and compared with hGMSCs. (JPEG 769 kb)

Additional file 2: of Three-dimensional printed PLA scaffold and human gingival stem cell-derived extracellular vesicles: a new tool for bone defect repair

3D PLA and hGMSCs interactions. SEM micrographs at low (A) and high magnifications (B) showing ce... more

Bacterial Adherence to Two Suture Materials in Oral Surgery

A 10-year retrospective comparative human study on screw-retained versus cemented dental implant abutments

Journal of biological regulators and homeostatic agents, 2019

The aim of this 10-year retrospective study was to evaluate the long-term reliability, survival r... more The aim of this 10-year retrospective study was to evaluate the long-term reliability, survival rate and mechanical and biological complications of single-crown implant rehabilitations with two different types of fixture-abutment connections: screw-retained abutments (SRAs) with internal hexagonal connection, and cemented retained abutments (CRAs). A total of 300 single implant-supported crowns were analysed, which had been inserted between 2004 and 2007. Patients were classified according to two groups: the SRA group (n = 150) and the CRA group (n = 150). The primary outcome was marginal bone loss (MBL) on peri-apical radiographs. Bleeding on probing (BOP) and probing depth (PD) were also evaluated. Moreover, prosthetic complications were recorded. Analysis of variance (ANOVA) was used to evaluate the differences between the groups. The overall implant failure rate was 4.2%. The overall positive BOP index was 81.9% of the sites under investigation, as 83.4% for SRA and 80.4% for CR...

Graphene Oxide affects Staphylococcus aureus and Pseudomonas aeruginosa dual species biofilm in Lubbock Chronic Wound Biofilm model

Scientific Reports, 2020

Chronic wound management becomes a complex procedure because of the persistence of forming biofil... more Chronic wound management becomes a complex procedure because of the persistence of forming biofilm pathogens that do not respond to antimicrobial treatment. The aim of this paper is to detect the Graphene Oxide-GO effect on Staphylococcus aureus and Pseudomonas aeruginosa dual species wound biofilm in Lubbock Chronic Wound Biofilm-LCWB model. LCWB is a recognized model that mimics the spatial microbial colonization into chronic wounds and reproduces the wound and its clot. Staphylococcus aureus PECHA 10 and P. aeruginosa PECHA 4, are the pathogens used in the study. The GO effect on both in forming and mature biofilms, is detected by the evaluation of the CFU/mg reduction, the cell viability and ultrastructural analysis of the treated LCWBs. Graphene Oxide, at 50 mg/l, shows a significant antibiofilm effect in forming and mature LCWBs. In particular, during the biofilm formation, GO reduces the S. aureus and P. aeruginosa growth of 55.05% ± 4.73 and 44.18% ± 3.91 compared to the con...

format_quoteGO significantly inhibits biofilm formation, demonstrating promise as a novel tool for chronic wound management.format_quote

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Bone Microstructure Evaluation near Unloaded Dental Implants Combining Confocal Scanning Laser Microscopy, Circularly Polarized Light Microscopy, and Sem Backscattered Electrons Imaging

International Journal of Immunopathology and Pharmacology, 2007

The aim of this report is to present a new investigative approach to implant dentistry based on t... more The aim of this report is to present a new investigative approach to implant dentistry based on the correlation of qualitative and quantitative data reported on the same figure by overlapping different images collected on the specimen with different investigative systems. Six unloaded titanium dental implants retrieved with peri-implant bone from the mandible of 2 patients after a 6 month period were used in this study. Samples of the peri-implant tissues embedded in resin were imaged by scanning electron microscopy using backscattered electrons signal (SEM BSE), confocal scanning laser microscopy (CSLM) and circularly polarized light microscopy (CPLM). The SEM BSE images were used to identify the different levels of mineral density. The CSLM images provided all the information on cells and bone marrow spaces. The CPLM images gave the collagen fibre orientation. To overlap the images we used a program introduced by Alan Boyde, based on a linear transformation matrix which projects o...

format_quoteBone near unloaded implants showed comparable longitudinal and transverse collagen fiber areas with low mineral density close to implant surfaces.format_quote

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Multimodal-3D imaging based on μMRI and μCT techniques bridges the gap with histology in visualization of the bone regeneration process

Journal of tissue engineering and regenerative medicine, Jan 7, 2017

Bone repair/regeneration is usually investigated through x-ray computed microtomography (μCT) sup... more Bone repair/regeneration is usually investigated through x-ray computed microtomography (μCT) supported by histology of extracted samples, to analyze biomaterial structure and new bone formation processes. Magnetic Resonance Imaging (μMRI) shows a richer tissue contrast than μCT, despite at lower resolution, and could be combined with μCT in the perspective of conducting non-destructive 3D investigations of bone. A pipeline designed to combine μMRI and μCT images of bone samples is here described and applied on samples of extracted human jawbone core following bone graft. We optimized the co-registration procedure between μCT and μMRI images to avoid bias due to the different resolutions and contrasts. Furthermore, we used an Adaptive Multivariate Clustering, grouping homologous voxels in the co-registered images, to visualize different tissue types within a fused 3D metastructure. The tissue grouping matched the 2D histology applied only on one slice, thus extending the histology l...

Human periodontal ligament stem cells cultured onto cortico-cancellous scaffold drive bone regenerative process

European Cells and Materials, 2016

The purpose of this work was to test, in vitro and in vivo, a new tissue-engineered construct con... more The purpose of this work was to test, in vitro and in vivo, a new tissue-engineered construct constituted by porcine cortico-cancellous scaffold (Osteobiol Dual Block) (DB) and xeno-free ex vivo culture of human Periodontal Ligament Stem Cells (hPDLSCs). hPDLSCs cultured in xeno-free media formulation preserved the stem cells' morphological features, the expression of stemness and pluripotency markers, and their ability to differentiate into mesenchymal lineage. Transmission electron microscopy analysis suggested that after one week of culture, both noninduced and osteogenic differentiation induced cells joined and grew on DB secreting extracellular matrix (ECM) that in osteogenic induced samples was hierarchically assembled in fibrils. Quantitative RT-PCR (qRT-PCR) showed the upregulation of key genes involved in the bone differentiation pathway in both differentiated and undifferentiated hPDLSCs cultured with DB (hPDLSCs/DB). Functional studies revealed a significant increased response of calcium transients in the presence of DB, both in undifferentiated and differentiated cells stimulated with calcitonin and parathormone, suggesting that the biomaterial could drive the osteogenic differentiation process of hPDLSCs. These data were confirmed by the increase of gene expression of L-type voltage-dependent Ca 2+ (VDCC L ), subunits α1C and α2D1 in undifferentiated cells in the presence of DB. In vivo implantation of the hPDLSCs/DB living construct in the mouse calvaria evidenced a precocious osteointegration and vascularisation process. Our results suggest consideration of DB as a biocompatible, osteoinductive and osteoconductive biomaterial, making it a promising tool to regulate cell activities in biological environments and for a potential use in the development of new custom-made tissue engineering.

format_quoteScaffold presence enhances calcium channel expression in both undifferentiated and differentiated hPDLSCs, indicating osteoinductive properties.format_quote

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[Marginal adaptation after cementing of gold inlays cast by an experimental procedure. SEM analysis]

Minerva stomatologica, 2004

Marginal adaptation is one of the most important factors in dental reconstructions. The aim of th... more Marginal adaptation is one of the most important factors in dental reconstructions. The aim of the present work was to evaluate with in vivo and in vitro studies the marginal precision and the microstructure of the gold cast inlays. For the in vivo analysis 10 patients were selected, while for the in vitro study 10 extracted teeth were used. The impressions were made by polyvinylsiloxane while the master cast was made by gypsum type IV. The inlays were cast in gold (JRVT Jensen) using an experimental procedure. After cementing, the margins of each inlay was burnished. To evaluate the marginal discrepancy of in vivo inlays, the replica technique was used, while for the in vitro analysis the serial slice cut technique was performed. All samples were processed for metallographic analysis using SEM. The measured in vivo inlay marginal gap was 9,06+/-3,37 microns (mean+/-SD), while for in vitro inlay the marginal gap was of 10,47+/-2,09 microns (mean+/-SD). Metallographic analysis showed...

Peri-Implant Bone Resorption during Healing Abutment Placement: The Effect of a 0.20% Chlorhexidine Gel vs. Placebo—A Randomized Double Blind Controlled Human Study

BioMed Research International, 2018

Introduction. Peri-implant marginal bone loss (MBL) seems to be more pronounced in the first year... more Introduction. Peri-implant marginal bone loss (MBL) seems to be more pronounced in the first year of loading despite all the studies and changes implemented to reduce it. Among the different causes, the presence of a microgap makes the interface between fixture and abutment colonizable by bacteria, causing an inflammatory response and consequent bone resorption. To reduce this several local antiseptics like chlorhexidine digluconate (CHX) were used after surgical procedures. Aim. The objective was to radiologically compare the MBL when a 0.20% CHX gel or a placebo gel was applied to the implant-abutment interface during all surgical and prosthetic phases and for a follow-up period up to 12 months. Method. 32 patients (16 for each Group A and B) were enrolled and rehabilitated with a single implant (Cortex classic, Cortex, Shalomi, Israel). During each of the clinical stages a gel containing 0.20% CHX (Plak ®Gel; Polifarma Wellness Srl, Rome, Italy) or a placebo gel (Placebo, Polifar...

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Implant Periapical Lesion: Clinical and Histological Analysis of Two Case Reports Carried Out with Two Different Approaches

Bioengineering

Periapical implantitis (IPL) is an increasingly frequent complication of dental implants. The cau... more Periapical implantitis (IPL) is an increasingly frequent complication of dental implants. The causes of this condition are not yet entirely clear, although a bacterial component is certainly part of the etiology. In this case series study, two approaches will be described: because of persistent IPL symptoms, a patient had the implant removed and underwent histological analysis after week 6 from implantation. The histomorphometric examination revealed a 35% bone-implant contact area involving the coronal two-thirds of the implant. The apical portion of the fixture on the other hand was affected by an inflammatory process detectable on radiography as a radiolucent area. The presence of a probable root fragment, detectable as an imprecise radiopaque mass in the zone where the implant was later placed, confirms the probable bacterial etiology of this case of IPL. On the other hand, in case number 2, the presence of IPL around the fixture was solved by surgically removing the implant api...

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Radiographic and Histomorphologic Evaluation of the Maxillary Bone after Crestal Mini Sinus Lift Using Absorbable Collagen—Retrospective Evaluation

Dentistry Journal

Background: After tooth extraction, the alveolar bone loses volume in height and width over time,... more Background: After tooth extraction, the alveolar bone loses volume in height and width over time, meaning that reconstructive procedures may be necessary to perform implant placement. In the maxilla, to increase the bone volume, a mini-invasive surgery, such as a sinus lift using the crestal approach, could be performed. Methods: A crestal approach was used in this study to perform the sinus lift, fracturing the bone and inserting collagen (Condress®). The single dental implant was placed in the healed bone after six months. Results: The newly formed bone was histologically analyzed after healing. Histomorphological analyses confirmed the quality of the new bone formation even without graft biomaterials. This is probably due to the enlargement of the space, meaning more vascularization and stabilization of the coagulum. Conclusion: Using just collagen could be sufficient to induce proper new bone formation in particular clinical situations, with a minimally invasive surgery to perfo...

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Clinical Performances and Biological Features of Clear Aligners Materials in Orthodontics

Frontiers in Materials, 2022

In recent years, aesthetic concerns regarding orthodontic appliances have significantly increased... more In recent years, aesthetic concerns regarding orthodontic appliances have significantly increased due to the growing number of adult patients undergoing orthodontic therapy. Clear removable aligners have seen growing popularity as an aesthetic and comfortable alternative to traditional fixed appliances. Clear aligner therapy also appears more respectful of the patient’s periodontal health; in fact, clear aligners allow the patients to maintain proper daily oral hygiene thanks to being removable. Among the parameters that affect the clinical efficacy of aligners, the material employed for their manufacturing plays a key role. The present paper aims to review the most used materials in manufacturing clear aligners, focusing on their clinical and mechanical performances, according to the current state of literature. Furthermore, biological features of the different materials are also examined regarding their effects on dental and periodontal tissues, oral mucosa, and potential systemic...

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Lithium disilicate and zirconia reinforced lithium silicate glass-ceramics for CAD/CAM dental restorations: biocompatibility, mechanical and microstructural properties after crystallization

Journal of Dentistry, 2022

OBJECTIVES The objective of this study was to define the impact of heating rate on the crystal gr... more OBJECTIVES The objective of this study was to define the impact of heating rate on the crystal growth, the mechanical properties, and the biocompatibility of three different kinds of CAD/CAM glass-ceramics treated with a conventional furnace. METHODS Lithium disilicate (IPS EMax-CAD, Ivoclar Vivadent) (LS2) and two zirconia reinforced lithium silicate (ZLS) ceramics (Vita Suprinity PC, VITA Zahnfabrik; Celtra Duo, Dentsply Sirona) (ZLSS; ZLSC) were used. The mechanical properties and the crystal growth were evaluated on 42 specimens (n=14 per group). The thermal treatments recommended by the manufacturers were carried out. All groups were tested for fracture toughness (Ft) and Vickers hardness (Hv). Scanning electron microscope (SEM) images were taken after a slight surface etching with hydrofluoric acid solution (1% for 20 sec). Differential Thermal Analysis (DTA) was performed and cellular adhesion with human periodontal ligament stem cells (hPDLSCs) culture was qualitatively assayed. Data were analysed with Repeated Measurements ANOVA and ANOVA followed by Tukey post hoc test. RESULTS The crystals' mean size (±SD) after heat treatment was 1650.0 (±340.0) nm for LS2, 854.5 (±155.0) nm for ZLSS and 759.9 (±118.4) nm for ZLSC (p<0.05 among the groups). As consequence of crystallization, the Hv was 6.1±0.3 GPa for LS2, 7.6±0.7 GPa for ZLSS and 7.1±0.5 GPa for ZLSC (p<0.05 for LS2 vs ZLSS and ZLSC), while the Ft was 2.2±0.1 MPa m1/2 for LS2, 4.7±0.8 MPa m1/2 for ZLSS and 3.8±0.6 MPa m1/2 for ZLSC (p<0.05 among the groups). The DTA curves showed a crystallization process for LS2, ZLSS and ZLSC at a temperature range 810°C to 840°C. The amount of adherent hPDLSCs was superior on LS2 than on ZLS. CONCLUSIONS All the CAD/CAM materials can be properly crystallized if heat treated following the manufacturers' instructions. The crystallization process highly depends on temperature. ZLS glass ceramics show significantly inferior crystals dimensions and higher fracture toughness and Vickers hardness than LS2 ceramic. hPDLSCs cultured on LS2 have a superior adhesion than those cultured on ZLS. CLINICAL SIGNIFICANCE The clinical interest of this study relies on the demonstration that a proper heat-treatment of CAD/CAM lithium disilicate and ZLS glass ceramics generates products that are suitable for clinical service. The differences highlightable in mechanical properties and biocompatibility behaviour do not affect their successful clinical application.

Additional file 4: of Three-dimensional printed PLA scaffold and human gingival stem cell-derived extracellular vesicles: a new tool for bone defect repair

Table S1. The differential gene expression between 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGM... more Table S1. The differential gene expression between 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGMSCs compared with hGMSCs is given as expression value and fold change expressed in logarithm with base 2 (FC Log2). Gene ontology (GO) processes indicate the gene classification in the "regulation of ossification" and "ossification". Instead, the statistical significance is indicated by the false discovery rate (FDR), q values ≤ 0.05 were considered statistically significant. Table S2. The differential gene expression between 3D-PLA+ EVs + hGMSCs and 3D-PLA+ PEI-EVs + hGMSCs compared with hGMSCs is given as expression value and fold change expressed in logarithm with base 2 (FC Log2). Gene ontology (GO) processes indicate the gene classification in the "regulation of osteoblast differentiation" and "osteoblast differentiation". Instead, the statistical significance is indicated by the false discovery rate (FDR), q values ≤ 0.05 were considered stat...

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