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2D DNA lattice arrays assembled from DNA dumbbell tiles using poly(A-T)-rich stems

Nanoscale, Nov 28, 2019

Enzymes of T4 DNA ligase and Exonuclease-I were purchased from Takara Biotechnology Co., Ltd. (Da... more Enzymes of T4 DNA ligase and Exonuclease-I were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Mica sheets for AFM imaging were from Nanjing Zhongjingkeyi Technology Co., Ltd. Water (18 MΩ cm) was from Milli-Q Ultrapure Water Purification System. Ethylenediaminetetraacetate (EDTA), urea, magnesium acetate tetrahydrate, boric acid, acetic acid, and tris(hydroxymethyl)aminomethane (Tris) were purchased from Sigma Aldrich Corporation. The TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), TBE buffer (89 mM Tris, 89 mM boric acid, and 2 mM EDTA, pH 8.3), and TAE-Mg buffer (40 mM Tris, 40 mM HAc, 2 mM EDTA and 12.5 mM Mg(Ac)2, pH 6.0 ± 0.5) were freshly prepared. 2. Methods and Instruments 2.1 Gel electrophoresis was carried out on a polyacrylamide electrophoresis plate (length × width × depth= 150 mm × 100 mm × 1.5 mm) system from Beijing Liuyi Instrument Factory. The running buffer for the denaturing PAGE is TBE, and that for the native PAGE is TAE-Mg. After gel electrophoresis, the gel was dyed with GelRed TM about 30 min and analysed under UV light. The gel documentation and analysis were carried out with Image J. 2.2 UV-Vis measurement of DNA concentrations was performed with a Nanodrop 2000 (Thermo). 2.3 AFM imaging was carried out on a Fastscan AFM (Bruker Corp.) with ScanAsyst-Fluid+ tips under the PeakForce Tapping mode in fluid, the sample solution (2 µL) was deposited onto a freshly cleaved mica surface and left stay for 2 min. Then 80 μL TAE-Mg buffer was added on top of the sample and an extra 40 μL of the same buffer was added onto the AFM tip.

format_quoteDNA precursor mixtures were prepared at 3.5 μM for ligation, utilizing T4 DNA ligase for enhanced DNA assembly.format_quote

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Identification and determination of phylogenetic relationship among Labeo rohita, Labeo catla and their reciprocal hybrids by traditional and molecular tools

Aquaculture Research

Effect of acclimated temperature on thermal tolerance, immune response and expression of HSP genes in Labeo rohita, Catla catla and their intergeneric hybrids

Journal of thermal biology, 2020

The ability of a species and population to respond to a decrease or an increase in temperature de... more The ability of a species and population to respond to a decrease or an increase in temperature depends on their adaptive potential. Here, the critical thermal tolerance (CTmax and CTmin) of four populations: Labeo rohita, Catla catla, and their reciprocal hybrids L. rohita♀× C. catla♂ (RC) and C. catla♀ × L. rohita♂ (CR) being acclimatized at four acclimation temperatures (22, 26, 30 and 34 °C) were determined. All populations indicated substantial variations (P < 0.05) in CTmax and CTmin values. L. rohita displayed, comparatively the highest CTmax with largest total and intrinsic polygon zones as well as the upper and lower acquired thermal tolerance zones followed by RC and CR hybrids, while C. catla showed significantly the highest CTmin value and the smallest intrinsic and acquired thermal tolerance zones. Both hybrids illustrated low parent heterosis (≤11%). Additionally, the highest expression of Hsp70 and Hsp90 (heat shock proteins) genes, serum lysozyme level, respiratory...

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