Salinity stress in soil arises when high salt levels, especially sodium chloride, hinder plant gr... more Salinity stress in soil arises when high salt levels, especially sodium chloride, hinder plant growth by disrupting water and nutrient uptake. Panchagavya is a fermented preparation made up of five cow products (milk, dung, urine, curd, and ghee) used in natural farming. This study aims to investigate the beneficial effects of Bacillus safensis PG-54, a salt-tolerant bacteria isolated from fermented panchagavya on the growth and development of Sorghum bicolor under salinity stress. All plant growth-promoting activities, seed germination, and pot studies were conducted using 1.4% w/v NaCl treatment along with a control. The Bacillus safensis PG-54 showed better plant growth-promoting traits like indole-acetic acid (73.98 μg/ml), gibberellic acid (3.44 mg/ml), ammonia (37.53 μmol/ml), phosphate solubilization (88.76 μg/ml), and exopolysaccharide production (1.33 g/L). Bacterial culture treated seeds showed 87% germination and a 113.1 seed vigour index. The addition of B. safensis PG-54 culture enhanced root length (6.01 ×), shoot length (1.37 ×), leaf length (1.71 ×), wet weight (1.17 ×), and dry weight (1.73 ×) on sorghum plants in salinity stress. Biochemical parameters were also improved after application of B. safensis PG-54 like chlorophyll (9.54 mg/g tissue), protein (0.82 μg/ml), sugar (1.91 μg/ ml), phenolics (3.70 μg/ml), proline (0.18 μg/ml) and salt tolerance index (0.711). The present study demonstrates the efficacy of Bacillus safensis PG-54 in improving plant growth and salt stress resilience, offering a promising solution for sustainable agriculture in saline soil.
Unveiling the Potential of Bacillus Safensis Pg-54 Isolated from Fermented Panchagavya on Sorghum Bicolor in Salinity Stress
A review on recent upgradation and strategies to enhance cyclodextrin glucanotransferase properties for its applications
International Journal of Biological Macromolecules, Dec 31, 2023
Statistical optimization of medium components for enhanced acetoin production from molasses and soybean meal hydrolysate
Applied Microbiology and Biotechnology, 2006
The nutritional requirements for acetoin production by Bacillus subtilis CICC 10025 were optimize... more The nutritional requirements for acetoin production by Bacillus subtilis CICC 10025 were optimized statistically in shake flask experiments using indigenous agroindustrial by-products. The medium components considered for initial screening in a Plackett–Burman design comprised a-molasses (molasses submitted to acidification pretreatment), soybean meal hydrolysate (SMH), KH2PO4·3H2O, sodium acetate, MgSO4·7H2O, FeCl2, and MnCl2, in which the first two were identified as significantly
Statistical optimization of medium components for biosurfactant production by <i>Pseudomonas guguanensis</i> D30
Preparative Biochemistry & Biotechnology, Oct 10, 2021
Abstract Biosurfactant production by Pseudomonas guguanensis D30 was reported using mineral oil i... more Abstract Biosurfactant production by Pseudomonas guguanensis D30 was reported using mineral oil in submerged condition. Twelve medium components were tested at two levels by Plackett–Burman design, among them, mineral oil, yeast extract, peptone, MgSO4, and CaCl2 found significant on the basis of emulsification index. These five significant components were further optimized through central composite design (CCD). The experimental design was successfully used for regression analysis and the significant model suggested the solution of 10% (v/v) mineral oil, 3.0 g/L (w/v) yeast extract and 0.2 g/L (w/v) peptone for 13.14 g/L predicted biosurfactant production. We kept the suggested concentrations of medium components and got 13.34 ± 0.08 g/L biosurfactant production, which is almost double the conventional one-factor-at-a-time production (7.126 ± 0.12 g/L). It reduced the surface tension of the medium up to 28 ± 1.2 mN/m. We found ethyl acetate a suitable solvent for biosurfactant extraction amongst methanol, chloroform, and methanol:chloroform. The partially purified biosurfactant was chemically characterized as lipopeptide by Fourier transform infrared spectroscopy (FT-IR). Graphical Abstract
Cyclodextrin glucanotransferase: fundamentals and biotechnological implications
Microbial surfactants are amphiphilic surface-active substances aid to reduce surface and interfa... more Microbial surfactants are amphiphilic surface-active substances aid to reduce surface and interfacial tensions by accumulating between two fluid phases. They can be generically classified as low or high molecular weight biosurfactants based on their molecular weight, whilst overall chemical makeup determines whether they are neutral or anionic molecules. They demonstrate a variety of fundamental characteristics, including the lowering of surface tension, emulsification, adsorption, micelle formation, etc. Microbial genera like Bacillus spp., Pseudomonas spp., Candida spp., and Pseudozyma spp. are studied extensively for their production. The type of biosurfactant produced is reliant on the substrate utilized and the pathway pursued by the generating microorganisms. Some advantages of biosurfactants over synthetic surfactants comprise biodegradability, low toxicity, bioavailability, specificity of action, structural diversity, and effectiveness in harsh environments. Biosurfactants a...
Biosurfactants are the biologically synthesized surface-active agents. They are amphiphilic compo... more Biosurfactants are the biologically synthesized surface-active agents. They are amphiphilic compounds consisting of hydrophilic and hydrophobic domains, that award the organism the ability to accumulate between fluid phases thus reducing surface and interfacial tension. Biosurfactants are produced by a number of microorganisms which include Bacillus sp., Rhodococcussp., Candida sp., Pseudomonas aeruginosa. They are classified based on their molecular weight and chemical composition. Several advantages of biosurfactants over the chemical surfactants are biodegradability, low toxicity, simplicityand bioavailability, specificity of action, structural diversity and effectiveness at extreme conditions. The physiological functions of biosurfactant production in microorganisms includes activities like, antimicrobial, anti-adhesive, antioxidant and the capability to compose substrates readily accessible for uptake by the cells in unfavorable environmental circumstances. Biosurfactants have ...
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