Papers by Andreas Blennow
Food Hydrocolloids, 2018
Protein-starch interaction. Control: Extruded wheat flour (black); Treatment 1: extruded blend of... more Protein-starch interaction. Control: Extruded wheat flour (black); Treatment 1: extruded blend of native pea protein and wheat flour (blue) and treatment 2: extruded blend of hydrolyzed pea protein and wheat flour (magenta). The magnitude of interaction found to be greatest in the blend with hydrolysed protein through hydrogen bonding. Hydrolysed pea proteins mitigate in vitro wheat starch digestibility
Journal of Cereal Science, 2017
Users may download and print one copy of any publication from the public portal for the purpose... more Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Carbohydrate Polymers, 2015
Users may download and print one copy of any publication from the public portal for the purpose... more Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Nano-crystal platelets of A- and B-type starches. A preliminary study
ABSTRACT
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RESEARCH ARTICLE The Role of Cysteine Residues in Redox Regulation and Protein Stability of Arabidopsis thaliana Starch Synthase 1
Starch biosynthesis in Arabidopsis thaliana is strictly regulated. In leaf extracts, starch synth... more Starch biosynthesis in Arabidopsis thaliana is strictly regulated. In leaf extracts, starch synthase 1 (AtSS1) responds to the redox potential within a physiologically relevant range. This study presents data testing two main hypotheses: 1) that specific thiol-disulfide exchange in AtSS1 influences its catalytic function 2) that each conserved Cys residue has an impact on AtSS1 catalysis. Recombinant AtSS1 versions carrying combinations of cys-teine-to-serine substitutions were generated and characterized in vitro. The results demon-strate that AtSS1 is activated and deactivated by the physiological redox transmitters thioredoxin f1 (Trxf1), thioredoxinm4 (Trxm4) and the bifunctional NADPH-dependent thior-edoxin reductase C (NTRC). AtSS1 displayed an activity change within the physiologically relevant redox range, with a midpoint potential equal to-306 mV, suggesting that AtSS1 is in the reduced and active form during the day with active photosynthesis. Cys164 and Cys545 were the ke...
The effects of molecular fine structure on rice starch granule gelatinization dynamics as investigated by in situ small-angle X-ray scattering
Food Hydrocolloids, 2021
Abstract Dynamic changes of rice starch granules selected for different amylose contents were ana... more Abstract Dynamic changes of rice starch granules selected for different amylose contents were analyzed in excess water while heated in-situ in a small-angle X-ray scattering (SAXS) instrument. Normal rice starch (NS) and rice starch with high amylopectin (HAP) and high amylose (HAM) were used as models. A 1D linear correlation function and a combination power-law and Gaussian function were used to extract the starch lamellar structure parameters and the fractal dimension, ordering and distribution of starch lamellae from SAXS data. For the resulting starch paste/gels, a model of two-correlation length was fitted to afford the correlation length (ξ) for the paste/gel system. The results showed that HAM exhibited higher long period (LP) and thickness of the crystalline layers (dc) values than HAP and NS. However, HAP showed the highest ordering lamellar structure. HAP granules were more thermostable than the amylose containing starches. For the gelatinized starches, HAM showed the highest correlation length values but these notably decreased with increasing temperature indicating strong chain segment interaction. This research reveals essential structural changes in lamellae of rice starch granules and rice starch gel structure, which provides potentially useful in the working of starch-based foods and materials.
Carbohydrate Polymers, 2020
The relationship between the expression pattern of starch biosynthesis enzymes and molecular stru... more The relationship between the expression pattern of starch biosynthesis enzymes and molecular structure of high amylose maize starch Yuyue Zhong (Conceptualization) (Investigation) (Methodology) (Software) (Writing-original draft) (Writing-review and editing), Linsan Liu (Investigation) (Data curation), Jianzhou Qu (Software), Silu Li (Software), Andreas Blennow (Writing-original draft) (Writing-review and editing), Shayakhmetova Altyn Seytahmetovna (Formal analysis), Xingxun Liu (Resources) (Conceptualization) (Funding acquisition) (Supervision), Dongwei Guo (Resources) (Conceptualization) (Funding acquisition) (Supervision)
Frontiers in Plant Science, 2018
Metabolism of starch is a major biological integrator of plant growth supporting nocturnal energy... more Metabolism of starch is a major biological integrator of plant growth supporting nocturnal energy dynamics by transitory starch degradation as well as periods of dormancy, regrowth, and reproduction by utilization of storage starch. Especially, the extraordinarily well-tuned and coordinated rate of transient starch biosynthesis and degradation suggests the presence of very sophisticated regulatory mechanisms. Together with the circadian clock, land plants (being autotrophic and sessile organisms) need to monitor, sense, and recognize the photosynthetic rate, soil mineral availability as well as various abiotic and biotic stress factors. Currently it is widely accepted that posttranslational modifications are the main way by which the diel periodic activity of enzymes of transient starch metabolism are regulated. Among these mechanisms, thiol-based redox regulation is suggested to be of fundamental importance and in chloroplasts, thioredoxins (Trx) are tightly linked up to photosynthesis and mediate light/dark regulation of metabolism. Also, light independent NADP-thioredoxin reductase C (NTRC) plays a major role in reactive oxygen species scavenging. Moreover, Trx and NTRC systems are interconnected at several levels and strongly influence each other. Most enzymes involved in starch metabolism are demonstrated to be redox-sensitive in vitro. However, to what extent their redox sensitivity is physiologically relevant in synchronizing starch metabolism with photosynthesis, heterotrophic energy demands, and oxidative protection is still unclear. For example, many hydrolases are activated under reducing (light) conditions and the strict separation between light and dark metabolic pathways is now challenged by data suggesting degradation of starch during the light period.
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Papers by Andreas Blennow