Papers by Prof. Ayman A Diab
In the present study, the effect of methanol extracts of two plant species, Dizygotheca kerchovea... more In the present study, the effect of methanol extracts of two plant species, Dizygotheca kerchoveana (Maliaceae) and Azadirachta indica (Araliacaea) were tested on the activity of Schistosoma mansoni worms. Efficacy of two plant extracts compared with praziquantel (PZQ) was evaluated in vivo. The results showed that reduction was 90% in case of PZQ treatment compared to 76.91%, 62.64% after treatment with D. kerchoveana and A. indica extracts respectively. PZQ recorded highest significant number of dead ova into oogram pattern (80±1.80) at 5wk PI. But, good results were obtained by of D. kerchoveana and A. indica methanol extracts (65.00±4.05 & 60.60±3.60, respectively) at 3wk PI. Efficacy of the plant extract showed a significant (P<0.01) reduction in ova to 91-96.20% in intestine, 83.36-91.24% in hepatic tissues as compared to PZQ (10-66.50% & 1.14-80.64%, respectively). Reduction in hepatic granulomadiameter at 3wk PI was significantly (P<0.01) reduced in group treated with ...
Materials, 2020
There is a pressing demand to synthesize polymers that have antibacterial and antifungal properti... more There is a pressing demand to synthesize polymers that have antibacterial and antifungal properties. The aim of this study was to synthesize a crosslinked hydrophilic terpolymer with acrylamide, acrylonitrile, acrylic acid, acrylamido-2-methylpropane sulphonic acid and ethylene glycol dimethacrylate as a crosslinker. The chemical structure and thermal stability of the prepared cross-linked terpolymers were confirmed by spectroscopic and thermal analyses. Moreover, the swelling experiments were performed to investigate their swelling capacity. Furthermore, the efficiency of the synthesized cross-linked polymer gels was assessed as an antimicrobial agent against Gram-positive, Gram-negative bacteria and fungal strains. The synthesized polymers showed broad inhibition effect, with more antibacterial activity by the AM4 polymer sample containing high percentage of acrylonitrile monomer in the prepared terpolymers (4 mol ratio of acrylic acid: 1 mol ratio of acrylamide: 16 mole ratio of ...
Asian Pacific journal of cancer prevention : APJCP, Nov 26, 2017
HCV induced hepatitis and hepatocellular carcinoma as its sequel are major health problems world-... more HCV induced hepatitis and hepatocellular carcinoma as its sequel are major health problems world-wide and especially in Egypt. For diagnosis and during treatment of liver diseases, liver functions are monitored through determination of serum levels of liver enzymes and α-fetoprotein although the obtained information is generally not sufficient for either early detection of hepatic insult or effective follow up of therapeutic effects. More sensitive biomarkers may help to achieve these goals. MiRNAs are small non-coding RNAs that have an important role in gene expression and regulation. Many, such as miR-224, miR-215, miR-143 are correlated with tumor appearance and with the degree of fibrosis in lung, breast and colon cancer. This study was performed to estimate the level of these miRNAs in serum of patients with HCV-associated hepatitis and HCC in relation to grade of hepatitis, stage of fibrosis and differentiation of tumor tissue. In addition, correlations between serological and...
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology, 2017
INTRODUCTION Food additives are substances that are added to food to extend the time of using by ... more INTRODUCTION Food additives are substances that are added to food to extend the time of using by retarding or inhibiting growth of microorganisms. Also, they have other uses including coloring, sweetening, flavouring, and thickening (Rekha and Dharman, 2011). For a long time, no observed adverse effects level have been proved on the basis of toxicological studies. Recently, studies proved that the consumption of some processed foods by additives might have increased the risk of cancer in human although the respected legal limits of these additives by the manufactures. The increased carcinogenicity risk caused by food additives can be due to various factors; interaction of food additives with some food ingredients, food processing may change the chemical formula of food additives to a formula like carcinogenic compound, a negative synergistic effects when combined with other additives, unsuitable storage conditions, and unknown carcinogenic by-products occurring during the food processing (Gülsoy et al., 2015). Sodium sulphite can induce inhibition of DNA synthesis in Vicia faba root, moreover, bridges in anaphase and interphase chromatin erosion nuclei ((Njagi and Gopalan , 1982). Benzoic acid did not induce genetic activity in tests of mammalian ARTICLE INFO
GM Crops & Food, 2013
Recent advances in genomics research has provided new tools that will assist breeders in their br... more Recent advances in genomics research has provided new tools that will assist breeders in their breeding programs for crop improvement specially when dealing with complex genetic traits or quantitively inhertied traits. Most agronomic traits of cotton are quantitatively inherited and do not consistently fall into discrete classes because the environmental conditions greatly modify their performance. Cotton is the world's leading natural fiber and second most important oilseed crop and has been a focus of genetic, systematic and breeding research. The genetic and physiological bases of some important agronomic traits in cotton were investigated by QTL mapping through constructing of genetic map with chromosomal assignment. A segregating F2 population derived from an interspecific cross (G. barbadense x G. hirsutum) between two genotypes, cvs. 'Giza 83' and "Deltapine" was used in this study. Different molecular markers including ssR, esT, esT-ssR, AFLp and RApD were employed to identify markers that reveal differences between the parents. In total 42 new markers were merged with 140 previously mapped markers to produce a new map with 182 loci covering a total length of 2370.5 cM. Among these new markers, some of them were used to assign chromosomes to the produced 26 linkage groups. The LG2, LG3, LG11 and LG26 were assigned to chromosomes 1, 6, 5 and 20 respectively. single point analysis was used to identify genomic regions controlling traits for plant height, number of nodes at flowering time, bolling date, days to flowering and number of bolls. In total 40 significant QTL were identified for the five traits on 11 linkage groups (1, 2, 3, 4, 5, 10, 11, 12, 18, 19 and 23). This work represents an improvement of the previously constructed genetic map in addition to chromosomal assignment and detection of new significant QTL for the five traits in egyptian cotton. The significant QTLs detected in this study can be employed in marker assisted selection for molecular breeding programs aiming at developing cotton cultivars with improved agronomic traits.
Molecular Biology Reports, 2011
Trehalose is a non-reducing disaccharide which consists of two glucose units that functions as a ... more Trehalose is a non-reducing disaccharide which consists of two glucose units that functions as a compatible solute to stabilize the membrane structures under heat and desiccation stress. Trehalose-6phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) are the key enzymes for trehalose biosynthesize in the plant kingdom. On the basis of bioinformatics prediction, fragment containing an open reading frame of 945 bp was cloned from durum wheat. Sequence comparison and analysis of conserved domains revealed the presence of a TPP domain. Full length of the gene was isolated using gene race technology. Semi-quantitative RT-PCR and real time quantitative PCR indicated that the expression of this gene is up-regulated in response to drought stress. The biochemical assay of the trehalase activity showed that the enzyme's activity decreased under the dehydration stress. The obtained phylogenic tree showed that the isolated TPP protein forms a distinct clad close to the Oryza sativa trehalose-6-phosphate phosphatase. In silico and comparative mapping indicated that the isolated TPP gene is localized on rice chromosome 8, durum wheat chromosome 20, bread wheat chromosome 3B, oat linkage group E, sorghum chromosome 4 and barley 5H.
The Planning of Emergency Seed Supply for Afghanistan in 2002 and Beyond: a draft concept paper prepared for the Tashkent Conference
&amp;amp;amp;amp;amp;quot;Findings and recommendations. 1. Scope of cereal seed aid needed in... more &amp;amp;amp;amp;amp;quot;Findings and recommendations. 1. Scope of cereal seed aid needed in 2002. Assessments of village-level seed aid requirements are urgently needed in cereal-growing areas. Afghani farmers may need about 80,000 MT of wheat seed aid in 2002 of which—by as yet untested assumptions--20,000 MT would be needed in irrigated areas and 60,000 MT in rainfed areas. Rice, maize, and barley seed aid requirements may be on the order of 8000, 7000, and 7000 MT, respectively. Both actual needs and ability to deliver may be much lower. 2. Local versus imported procurement. For rainfed wheat seed aid, an appropriate locally-based strategy to supply 60,000 MT in 2002 may be simply impossible, but perhaps it could take the form of: a) redistribution of existing stocks of seed, including unmilled grain still stored as a single variety with good germination; 15,000 MT; b) replication of rainfed variety seed, including Dayima 94, Ghori 94, and local landraces (subject to vernalization &amp;amp;amp;amp;amp;amp; photoperiod issues) in irrigated fields this spring to produce 35,000 MT; c) import of international variety seed identical or (similar?) to Lalmi 1, Lalmi 2 and Lalmi 3; 10,000 MT. For the hypothetical 20,000 irrigated wheat seed aid, perhaps it would be possible to import for the spring planting 10,000 MT of modern variety seed from Pakistan and elsewhere for distribution only in areas where it has already been found to be well adapted. And for the other 10,000 MT, relying on the existing FAO-led seed production network and the hiring of additional skilled farmers to produce about 10,000 MT of quality seed for redistribution to farmers. 3. Strengthening of the formal seed sector in Afghanistan including the top end of the seed chain. Further programs involving CGIAR centers and National and Foreign University breeding programs would complement the existing seed network created by NGOs and the FAO-Afghanistan Seed Program. Support and capacity building should target both the formal and informal seed sectors and the public and private seed sectors in Afghanistan. Review of crop germplasm collected in Afghanistan over the past 80 years and of exotic crop germplasm tested in Afghanistan over the past 40 years would provide a stronger foundation for the planning of new breeding and crop improvement programs in country. Provision for training of Afghani scientists in the area of seed technology to the graduate degree level would be important to this longer-term undertaking. In the short term, tendering for seed supply at the provincial level coupled with oversight and training of contract farmers may help the more competent contract farmers to upgrade to the status of Small Seed Enterprises.&amp;amp;amp;amp;amp;quot;
Plants, 2021
Biosorption is a bioremediation approach for the removal of harmful dyes from industrial effluent... more Biosorption is a bioremediation approach for the removal of harmful dyes from industrial effluents using biological materials. This study investigated Methylene blue (M. blue) and Congo red (C. red) biosorption from model aqueous solutions by two marine macro-algae, Ulva fasciata and Sargassum dentifolium, incorporated within acrylic fiber waste to form composite membranes, Acrylic fiber-U. fasciata (AF-U) and Acrylic fiber-S. dentifolium (AF-S), respectively. The adsorption process was designed to more easily achieve the 3R process, i.e., removal, recovery, and reuse. The process of optimization was implemented through one factor at a time (OFAT) experiments, followed by a factorial design experiment to achieve the highest dye removal efficiency. Furthermore, isotherm and kinetics studies were undertaken to determine the reaction nature. FT-IR and SEM analyses were performed to investigate the properties of the membrane. The AF-U membrane showed a significant dye removal efficiency...
Egyptian Journal of Genetics and Cytology, 2011
seudomonas fluorescens are gramnegative rod shaped bacteria that improve plant health and nutriti... more seudomonas fluorescens are gramnegative rod shaped bacteria that improve plant health and nutrition . Most studies describe P. fluorescens as a psychrotrophic bacterium unable to grow at temperatures greater
Materials, 2019
In the past few years, the development of hydrogel properties has led to the emergence of nanocom... more In the past few years, the development of hydrogel properties has led to the emergence of nanocomposite hydrogels that have unique properties that allow them to be used in various different fields and applications such as drug delivery, adsorption soil containing, tissue engineering, wound dressing, and especially antimicrobial applications. Thus, this study was conducted in order to fabricate a novel crosslinked terpolymer nanocomposite hydrogel using the free radical copolymerization method based on the usage of 2-acrylamido-2-methylpropane sulfonic acid (AMPS), acrylamide (AAm), acrylonitrile (AN), and acrylic acid (AA) monomers and iron oxide (Fe3O4) magnetic nanoparticles and using benzoyl peroxide as an initiator and ethylene glycol dimethacrylate (EGDMA) as a crosslinker. The structure of the synthesized composite was confirmed using Fourier transform infrared (FTIR) spectroscopy and x-ray powder diffraction (XRD) measurements. Furthermore, the surface morphology and the magn...
African Journal of Biotechnology, 2006
In the past, it has been difficult to accurately determine the location of many types of barley m... more In the past, it has been difficult to accurately determine the location of many types of barley molecular markers due to the lack of commonality between international barley linkage maps. In this study, a consensus map of barley was constructed from five different maps (OWB, VxHs, KxM, barley consensus 2 and barley consensus 2003) to produce the consensus AD-2005 map with 1536 markers. The QTL that have been identified in previous barley studies were then incorporated into the integrated consensus map to provide a quick method of aligning and comparing barley linkage maps and to identify markers closely linked to barley traits. The markers placed on this map are consistent with respect to order on the chromosomes with the individual maps and other barley maps with a few minor differences. The consensus AD-2005 was compared with rice Cornell RFLP map to examine the reliability of the constructed map in comparative genomic studies. Unlike previous consensus maps, the purpose of this consensus map (containing QTL) is to provide a tool for scientists to accurately locate molecular markers to chromosome regions responsible for economically important traits. It is estimated that markers placed on the consensus map are located very close to their true positions as determined by the five maps used in this study. It is envisaged that the consensus map will benefit small-grain researchers by providing an efficient means of choosing markers of interest and identifying QTL regions for future genetic or plant breeding studies on a worldwide basis.
Molecular Characterization of Some Egyptian Bread Wheat Genotypes
The main objective of this investigation was to assess the genetic diversity among five Egyptian ... more The main objective of this investigation was to assess the genetic diversity among five Egyptian bread wheat genotypes (Misr1 and Sids 13 cutlivars and the promising lines No.1, No.2 and No.11 ) using SDS-PAGE and RAPD markers. The total number of SDS- bands was seven. Six SDS- bands were monomorphic while the other was polymorphic. Line-1 was characterized by the presence of band -3 with a molecular weight of 41.56 kDa. RAPD analysis showed that the number of polymorphic amplicons was 66 out of a total of 93 amplicons, thus revealing a level of 70.97 % polymorphism. The highest genetic similarity revealed by RAPD analysis (93.1%) was between Misr1 and Line 2 genotype. While, the lowest similarity (85.2 %) was between Line 1 and Line 2. The dendrogram separated Line1 from all the other genotypes. The four genotypes constituted a subcluster divided into two groups, one group composed of Misr 1 and Line 2, while the second group comprised Sids 13 and Line 11.
Identification and Functional Validation of Expressed Sequence Tags (ESTs) Preferentially Expressed in Response to Drought Stress in Durum Wheat
Drought is a major planetary stress problem that places greater limits on cereal productivity. Cr... more Drought is a major planetary stress problem that places greater limits on cereal productivity. Crop species belonging to the tribe Triticale represent the main foodstuff sources for humans. Because durum wheat (Triticum turgidum L.) shows better adaptation to semi-arid climates than bread wheat, its genome are likely to contain a pool of genes that are expressed for wide environmental adaptability. To investigate and understand the genetic response of drought tolerance in durum wheat, differentially expressed sequence tags (dESTs) were detected, characterized and cloned from durum wheat under different regimes of drought stress and the functions of some of these dESTs were determined. In this study, genes involved in drought stress at early stages of growth were profiled by a differential display technique from Egyptian durum wheat (Sohag 3). One, three, six, and 10 hours of drought stress were applied to produce 15 expressed cDNA fragments, which highlighted the genes that were exp...
Purification and immobilization of l-arginase from thermotolerant Penicillium chrysogenum KJ185377.1; with unique kinetic properties as thermostable anticancer enzyme
Archives of Pharmacal Research, 2014
L-Arginase, hydrolyzing L-arginine to L-ornithine and urea, is a powerful anticancer, L-arginine-... more L-Arginase, hydrolyzing L-arginine to L-ornithine and urea, is a powerful anticancer, L-arginine-depleting agent, against argininosuccinate synthase expressing tumors. Otherwise, the higher antigenicity and lower thermal stability of this enzyme was the main biochemical hurdles. Since, the intrinsic thermal stability of enzymes follow the physiological temperature of their producer, thus, characterization of L-arginase from thermotolerant Penicillium chrysogenum was the objective of this study. L-Arginase (Arg) was purified to its homogeneity from P. chrysogenum by 10.1-fold, with 37.0 kDa under denaturing PAGE, optimum reaction at 50 °C, pH stability (6.8-7.9), with highest molar ratio of constitutional arginine, glutamic acid, lysine and aspartic acid. The purified enzyme was PEGylated and immobilized on chitosan, with 41.9 and 22.1 % yield of immobilization. At 40 °C, the T1/2 value of free-Arg, PEG-Arg and Chit-Arg was 10.4, 15.6, 20.5 h, respectively. The free-Arg and Chit-Arg have a higher affinity to L-arginine (K m 4.8 mM), while, PEG-Arg affinity was decreased by about 3 fold (K m 15.2 mM). The inhibitory constants to the free and PEG-Arg were relatively similar towards HA and PPG. The IC50 for the free enzyme against HEPG-2 and A549 tumor cells was 0.136 and 0.165 U/ml, comparing to 0.232 and 0.496 U/ml for PEG-Arg, respectively. The in vivo T1/2 to the free Arg and PEG-Arg was 16.4 and 20.4 h, respectively as holo-enzyme. The residual L-arginine level upon using free Arg was 156.9 and 144.5 µM, after 6 and 8 h, respectively, regarding to initials at 253.6 µM, while for Peg-Arg the level of L-arginine was nil till 7 h of initial dosing. The titer of IgG was induced by 10-15 % in response to free-Arg after 28 days comparing to IgG titer for PEG-Arg.
Identification of drought-inducible genes and differentially expressed sequence tags in barley
Theoretical and Applied Genetics, 2004
Drought limits cereal yields in several regions of the world and plant water status plays an impo... more Drought limits cereal yields in several regions of the world and plant water status plays an important role in tolerance to drought. To investigate and understand the genetic and physiological basis of drought tolerance in barley, differentially expressed sequence tags (dESTs) and candidate genes for the drought response were mapped in a population of 167 F8 recombinant inbred lines derived from a cross between &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;Tadmor&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; (drought tolerant) and &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;Er/Apm&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; (adapted only to specific dry environments). One hundred sequenced probes from two cDNA libraries previously constructed from drought-stressed barley (Hordeum vulgare L., var. Tokak) plants and 12 candidate genes were surveyed for polymorphism, and 33 loci were added to a previously published map. Composite interval mapping was used to identify quantitative trait loci (QTL) associated with drought tolerance including leaf relative water content, leaf osmotic potential, osmotic potential at full turgor, water-soluble carbohydrate concentration, osmotic adjustment, and carbon isotope discrimination. A total of 68 QTLs with a limit of detection score &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; or =2.5 were detected for the traits evaluated under two water treatments and the two traits calculated from both treatments. The number of QTLs identified for each trait varied from one to 12, indicating that the genome contains multiple genes affecting different traits. Two candidate genes and ten differentially expressed sequences were associated with QTLs for drought tolerance traits.
BMC Plant Biology, 2013
A linkage map, primarily based on SSCP-SNP markers, was constructed using 188 F 2:3 mapping popul... more A linkage map, primarily based on SSCP-SNP markers, was constructed using 188 F 2:3 mapping population progenies produced from a cross between two pearl millet inbred lines having diverse parentage. The skeleton linkage map covered 1019 cM and it comprised of 44 markers distributed across the seven linkage groups. Average adjacent-marker intervals ranged from 14 cM on LG1 to 38 cM on LG6, with an overall mean of 23 cM. Using the F 2 linkage map and phenotypic data from the F 2 and F 2:3 generations of the mapping population, a total of 18 putative QTLs were detected for the three sink-size components. Eight QTLs explained 42.7% of observed phenotypic variation for panicle length using the F 2:3 data set. For panicle diameter, 5 QTLs explained 45.8% of observed phenotypic variation. Similarly for grain size, 5 QTLs explained 29.6% of phenotypic variation. Genomic regions associated with panicle length, panicle diameter, and grain size were comapped on LG6 between Xpsms88 and Xpsms2270, indicating the existence of a gene or gene cluster. The QTLs for panicle length on LG2 and LG6 (LOD > 3 in both F 2 and F 2:3 data sets), for panicle diameter on LG2 and LG3 (LOD > 14 in the F 2:3 data set), and for grain size on LG3 and LG6 (LOD > 3 in both F 2 and F 2:3 data sets) were identified as promising candidates for validation prior to possible application in marker-assisted breeding.
Cotton is the world's leading fiber crop and the second most important oil seed crop. In Egypt, p... more Cotton is the world's leading fiber crop and the second most important oil seed crop. In Egypt, plant breeders have made major contributions to the productivity of this crop; however, this has led to decreasing the genetic variation among Egyptian cotton varieties. Enhancing the productivity of cotton could be addressed through improving different agronomic traits including early flowering and maturation. In the present investigation, an interspecific cross (G. barbadense x G. hirsutum) was performed between two genotypes, Giza83 (late flowering) and Deltapine (early flowering) to develop F2 segregating population. Analysis of segregation among the 71 F2 individuals was performed using 3 RAPD, 10 SSR, 6 AFLP primer combinations. Twenty four AFLP primer combinations were used in bulked segregant analysis for flowering time. Linkage analysis and map construction were performed using Map Manager. The map showed 22 linkage groups with 140 markers covered a total length of 1556.7 cM. The average length of linkage groups ranged from 1.4 to 649.5. Single point analysis was used to identify the genomic regions controlling traits for plant height, number of nodes at flowering time, bolling date, days to flowering and number of bolls. In total, 30 significant QTL were identified for the five traits on ten linkage groups, among these 11 QTL for plant height, 8 for number of bolls, 4 QTL for each of days to flowering and bolling date and 3 QTL for number of nodes at flowering time. This work represents the first linkage map for the intercross between Giza83 and Deltapine showing chromosomal regions associated with some agronomic traits.
Effect of carboplatin and Nigella sativa oil on human breast cancer cells in vitro and Ehrlich ascites tumor bearing mice in vivo
Carboplatin is a synthetic antineoplastic agent used for cancer treatment and is considered to be... more Carboplatin is a synthetic antineoplastic agent used for cancer treatment and is considered to be the analogous of cisplatin. Nigella sativa oil is a herbaceous plant, it has been used for thousands of years for culinary and medical purposes. This study aimed to evaluate the effect of carboplatin and Nigella sativa oil alone and in combination together on human breast cancer cells (MCF-7) in vitro and Ehrlich ascites tumor bearing female mice (in vivo). The in vitro experiment on MCF-7 cells illustrated that IC50 of carboplatin was 11.8 μg/ml, also IC50 of Nigella sativa oil was 39 μg/ml on MCF-7 cells. In addition, IC50 of the combination between carboplatin and Nigella sativa oil was found to be 3.78 and 40 μg/ml, respectively. The in vivo experiment illustrated that carboplatin (10mg/kg) increased the enzyme activity of aspartate amino transferase (GOT) and aniline amino transferase (GPT)by 56.52% and 51.14%, respectively as compared to both healthy control (nontumor transplanted mice) and negative control. Also, the activity of GOT and GPT was increased by 14.75% and 19.84%, respectively as compared to healthy control under the effect of Nigella sativa oil (12ml/kg). While the activity of GOT and GPT was decreased as compared to negative control. The combination of carboplatin and Nigella sativa oil appeared to increase the enzyme activity of GOT and GPT by 62.41 and 49.39%, respectively as compared to both healthy control and negative control. Also, carboplatin induced DNA damage of liver tissue was performed by agarose gel electrophoresis and comet assay, while Nigella sativa oil showed intact DNA without any damage. The combination of carboplatin and Nigella sativa oil appeared to decrease the DNA damage as compared to carboplatin alone.
Characterization and optimization of magnetic Gum-PVP/SiO2 nanocomposite hydrogel for removal of contaminated dyes
Materials Chemistry and Physics
Molecular cloning, expression, sequence analysis and in silico comparative mapping of trehalose 6... more Molecular cloning, expression, sequence analysis and in silico comparative mapping of trehalose 6-phosphate gene from Egyptian durum wheat
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Papers by Prof. Ayman A Diab